Effects of stearic acid on the cryotolerance of the domestic cat (Felis silvestris catus) embryos

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Abstract

The current work aimed to study the effect of domestic cat (Felis silvestris catus) embryos in vitro exposure to saturated stearic acid (SA) and to evaluate how the change in lipid content affects the cryopreservation results. The addition of SA to the culture medium did not affect the development of cat embryos in vitro before cryopreservation. The total lipid amount in the SA-treated embryos was not changed as well. However, the lipid unsaturation degree was lower in embryos after in vitro exposure to SA. Moreover, the lipid phase transition onset temperature (T*) was higher in SA-treated embryos as compared with controls. These changes of intracellular lipids unsaturation degree and T* were associated with the impairment of embryo cryopreservation effectiveness. The results obtained may be of importance for the applying Genome Resource Banking concept to the Felinae species.

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E. Yu. Brusentsev

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences

Author for correspondence.
Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090

S. V. Okotrub

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences

Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090

D. A. Lebedeva

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University

Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090; prosp. Koptyuga 1, Novosibirsk, 630090; Pirogova 2, Novosibirsk, 630090

K. A. Okotrub

Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences

Email: amstis@yandex.ru
Russian Federation, prosp. Koptyuga 1, Novosibirsk, 630090

T. A. Rakhmanova

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University

Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090; prosp. Koptyuga 1, Novosibirsk, 630090; Pirogova 2, Novosibirsk, 630090

S. Ya. Amstislavsky1

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Institute of Automation and Electrometry, Siberian Branch of the Russian Academy of Sciences

Email: amstis@yandex.ru
Russian Federation, prosp. Lavrentyeva 10, Novosibirsk, 630090; prosp. Koptyuga 1, Novosibirsk, 630090

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2. Fig. 1. Experimental design. IVF – in vitro fertilization; LPL – lipid phase transition

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3. Fig. 2. Developmental stages of domestic cat (Felis silvestris catus) embryos after 66 hours of in vitro cultivation without (a, c) or with stearic acid –SA (d, e): early morulae (a, b) and late morulae (d, e) ); DAPI staining and fluorescence microscopy. White arrows – fragmentation, dotted arrows – spermatozoa. Scale – 50 µm

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4. Fig. 3. Effect of stearic acid (SA) on the degree of lipid unsaturation (top) and T* (bottom) in the cells of preimplantation embryos of the domestic cat (Felis silvestris catus). Data are presented as M ± SEM. Each point corresponds to one embryo. *p < 0.05; ***p < 0.001 compared to control

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5. Fig. 4. Fluorescence intensity of domestic cat (Felis silvestris catus) embryos stained with Nile red after 66 hours of in vitro cultivation without and with stearic acid (SA). a – number of photons (Me [Q1; Q3]), each point corresponds to one embryo; b, c-representative CLSM optical sections of domestic cat embryos stained with Nile red. b – without SC; with SK. Scale – 50 µm

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6. Fig. 5. Late morulae of the domestic cat (Felis silvestris catus) after freezing-thawing and subsequent cultivation in vitro for 30 hours (total time – 96 hours): control (a) and SC (b) groups; DAPI staining. Arrows indicate fragmented nuclei. Scale – 50 µm

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