Email this article Аnticoagulation Effects of Leucine-Containing Glyprolines in Hypercoagulation
- Authors: Grigorieva M.E.1, Obergan T.Y.1, Lyapina L.A.1
-
Affiliations:
- Lomonosov Moscow State University, Biology Faculty, Department of Human and Animal Physiology, Laboratory of Protective Blood Systems named after prof. B.A. Kudryashov
- Issue: No 6 (2023)
- Pages: 682-686
- Section: ФИЗИОЛОГИЯ ЖИВОТНЫХ И ЧЕЛОВЕКА
- URL: https://journals.rcsi.science/1026-3470/article/view/231708
- DOI: https://doi.org/10.31857/S1026347022600509
- EDN: https://elibrary.ru/LIIYUG
- ID: 231708
Cite item
Full Text
Abstract
The effects of two leucine-containing glyproline peptides Leu-Pro-Gly-Pro and Pro-Gly-Pro-Leu, including leucine from the N- and C-ends of the molecules, on the parameters of platelet (primary) and plasma hemostasis were studied. Peptides were administered intranasally for 5 days at a daily dose of 500 mcg/kg of body weight under conditions of hypercoagulation caused by intravenous administration of tissue thromboplastin to metabolic syndrome rats on high-calorie diet. Both peptides contributed to an increase in all types of fibrinolysis (total, enzymatic and non-enzymatic), anticoagulant activity and a decrease in platelet aggregation in rat blood plasma compared with the control. The maximum anticoagulant effect was established in a peptide containing leucine from the C-terminus.
About the authors
M. E. Grigorieva
Lomonosov Moscow State University, Biology Faculty, Department of Human and Animal Physiology, Laboratory of Protective Blood Systems named after prof. B.A. Kudryashov
Author for correspondence.
Email: mgrigorjeva@mail.ru
Russia, 119234, Moscow, Leninsky Gory, 1/12
T. Y. Obergan
Lomonosov Moscow State University, Biology Faculty, Department of Human and Animal Physiology, Laboratory of Protective Blood Systems named after prof. B.A. Kudryashov
Email: mgrigorjeva@mail.ru
Russia, 119234, Moscow, Leninsky Gory, 1/12
L. A. Lyapina
Lomonosov Moscow State University, Biology Faculty, Department of Human and Animal Physiology, Laboratory of Protective Blood Systems named after prof. B.A. Kudryashov
Email: mgrigorjeva@mail.ru
Russia, 119234, Moscow, Leninsky Gory, 1/12
References
- Баркаган З.С., Костюченко Г.И. Метаболически-воспалительная концепция атеротромбоза и новые подходы к терапии больных // Бюл. СО РАМН. 2006. № 2 (120). С. 132–138.
- Баркаган З.С., Момот А.П. Диагностика и контролируемая терапия системы гемостаза. М.: Ньюдиамед, 2008.
- Бышевский А.Ш., Галян С.Л., Калинин Е.П., Карпова И.А., Русакова О.А.,
- Ляпина Л.А., Григорьева М.Е., Оберган Т.Ю., Шубина Т.А. Теоретические и практические вопросы изучения функционального состояния противосвертывающей системы крови. М.: Адвансед Солюшнз, 2012. 160 с.
- Оберган Т.Ю., Григорьева М.Е. Исследование параметров гемостаза, липидного и углеводного обмена у крыс с модифицированной моделью экспериментального метаболического синдрома // Современные технологии в медицине. 2016. Т. 8. № 4. С. 303–305.
- Оберган Т.Ю., Рочев Д.Л. Противосвертывающие и антитромбоцитарные эффекты пептида Pro-Gly-Pro-Leu и лейцина при внутривенном введении животным // Сборник: Физиологическая активность регуляторных пептидов. M.: 2010. С. 67–68.
- Шабалина А.А., Ляпина Л.А., Рочев Д.Л., Костырева М.В., Танашян М.М., Суслина З.А. Гиполипидемические и фибриндеполимеризационные эффекты регуляторных лейцинсодержащих глипролинов в крови человека in vitro // Изв. РАН. Сер. биол. 2015. № 42 (1). С. 85−89.
- He X., Feng T., Zhang D., Zhuo R., Liu M. Identification and comprehensive analysis of the characteristics and roles of leucine-rich repeat receptor-like protein kinase (LRR-RLK) genes in Sedum alfredii Hance responding to cadmium stress // Ecotoxicol. Environ. Saf. 2018. № 167. P. 95−106.
- Ichimura N., Kasama T. Identification of valine- or leucine-containing glycopeptidolipids from Mycobacterium avium-intracellulare complex // Curr. Microbiol. 2012. V. 64. № 6. P. 561−568.
- Kong X., Zhang D.Y., Wu H.B., Li F.X. Losartan and pioglitazone ameliorate nephropathy in experimental metabolic syndrome rats // Biol. Pharm. Bull. 2011. V. 34. № 5. P. 693–698.
- López N., Sбnchez J., Palou A., Serra F. Gender-Associated Impact of Early Leucine Supplementation on Adult Predisposition to Obesity in Rats // Nutrients. 2018. V. 10. № 1. P. E76.
- Myasoedov N.F., Lyapina L.A., Grigorjeva M.E., Obergan T.Y., Shubina T.A., Andreeva L.A. Mechanisms for glyproline protection in hypercholesterolemia // Pathophysiology. 2016. V. 23. № 1. P. 27−33.
- Okabayashi H.F., Kanbe H.H., O’Connor C.J. The role of an L-leucine residue on the conformations of glycyl-L-leucine oligomers and its N- or C-terminal dependence: infrared absorption and Raman scattering studies // Euк. Biophys. J. 2016. № 1. P. 23−34.
- Watson T., Arya A., Sulke N., Lip G.Y.H. Antithrombotic therapy: relationship of indices of inflammation and thrombogenesis to arithmia burden in paroxysmal arterial fibrillation // Chest. 2010. V. 137. P. 869−876.
- Wilkinson D.J., Bukhari S.S.I., Phillips B.E., Limb M.C., Cegielski J., Brook M.S., Rankin D., Mitchell W.K., Kobayashi H., Williams J.P., Lund J., Greenhaff P.L., Smith K., Atherton P.J. Effects of leucine-enriched essential amino acid and whey protein bolus dosing upon skeletal muscle protein synthesis at rest and after exercise in older women // Clin. Nutr. 2017. № 17. P. 31340−31347.
