Synthesized Using β-Cyclodextrin Silver and Gold Nanoparticles as Radiosensitizers in Breast Cancer Radiotherapy
- Авторлар: Pustovalova M.V.1,2,3, Nekrasov V.D.1, Andreev E.V.3,4, Fadeikina I.N.3,4, Leonov S.V.1, Nechaev A.N.3,4, Osipov A.N.1,2,3
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Мекемелер:
- Moscow Institute of Physics and Technology
- A.I. Burnazyan Federal Medical Biophysical Center
- Joint Institute for Nuclear Research
- Dubna State University
- Шығарылым: Том 70, № 2 (2025)
- Беттер: 35-39
- Бөлім: Radiation Biology
- URL: https://journals.rcsi.science/1024-6177/article/view/361476
- DOI: https://doi.org/10.33266/1024-6177-2025-70-2-35-39
- ID: 361476
Дәйексөз келтіру
Толық мәтін
Аннотация
Purpose: 1) Synthesis and characterization of β-cyclodextrin stabilized silver and gold nanoparticles. 2) Evaluation of the effect of the synthesized nanoparticles on the severity of radiobiological effects in irradiated breast cancer (BC) cells.
Material and methods: Gold and silver nanoparticles were synthesized using β-cyclodextrin as a reducing agent and stabilizer. Human BC cell lines MDA-MB-231 (ER-/PR-/ EGFR+/ HER2-) and MCF7 (ER+/ PR+/ EGFR-/ HER2-) were used in the work. The cells were irradiated on a RUB RUST-M1 (Russia) X-ray irradiation facility, equipped with two X-ray emitters, at a dose rate of 0.85 mGy/min, 200 kV voltage, 5.0 mA current, 1.5 mm Al filter. Nanoparticles were added 24 h before irradiation at a concentration of 0.5 mg/l. Cells without nanoparticles were used as a control. To assess radiobiological effects, foci of the DNA damage marker protein (γH2AX) were analyzed 1 and 24 hours after irradiation. Statistical and mathematical data analysis was performed using GraphPad Prism 9.0.2.161 software (GraphPad Software). Statistical significance was assessed using analysis of variance (ANOVA).
Results: Effective radiosensitization of BC cell lines MDA-MB-231 and MCF7 using β-cyclodextrin stabilized gold and silver nanoparticles (AuNPs and AgNPs) was shown. The results obtained indicate the achievement of statistically significant results already at a concentration of 0.5 mg/l, which is at least 20 times less than the concentrations previously used to achieve a significant effect.
Негізгі сөздер
Авторлар туралы
M. Pustovalova
Moscow Institute of Physics and Technology; A.I. Burnazyan Federal Medical Biophysical Center; Joint Institute for Nuclear Research
Email: andreyan.radbio@gmail.com
Dolgoprudny, Russia; Moscow, Russia; Dubna, Russia
V. Nekrasov
Moscow Institute of Physics and Technology
Email: andreyan.radbio@gmail.com
Dolgoprudny, Russia
E. Andreev
Joint Institute for Nuclear Research; Dubna State University
Email: andreyan.radbio@gmail.com
Dubna, Russia
I. Fadeikina
Joint Institute for Nuclear Research; Dubna State University
Email: andreyan.radbio@gmail.com
Dubna, Russia
S. Leonov
Moscow Institute of Physics and Technology
Email: andreyan.radbio@gmail.com
Dolgoprudny, Russia
A. Nechaev
Joint Institute for Nuclear Research; Dubna State University
Email: andreyan.radbio@gmail.com
Dubna, Russia
A. Osipov
Moscow Institute of Physics and Technology; A.I. Burnazyan Federal Medical Biophysical Center; Joint Institute for Nuclear Research
Email: andreyan.radbio@gmail.com
Dolgoprudny, Russia; Moscow, Russia; Dubna, Russia
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