Molecular Variant Estimation of SLC11A1 Related Microsatellites in Chinese Indigenous Goats

详细

The aim of this study was to investigate the polymorphism of the SLC11A1 gene in Chinese indigenous goats. A total of 215 individuals from nine goat breeds were genotyped using two pairs of known SLC11A1. Thirty-four alleles were found in these breeds across two microsatellite loci. Observed heterozygosity ranged from 0.2292 ± 0.0607 in CDB to 0.3958 ± 0.0706 in CDM. The mean number of alleles ranged from 4.50 ± 2.12 in CDB to 7.50 ± 2.12 in CDM. The coefficient of inbreeding (F_IS) was lowest in CDB (0.267) and highest in DZ (0.459). All populations deviated from HWE in these two markers, except for JZ and CDM populations, which had one (EX-15). According to the F_ST results, only nine pairs were significantly different. Bayesian analysis using the program STRUCTURE, as well as Neighbor-joining tree analysis, both expressed 2 clusters, revealing genetic clustering patterns consistent with geographic location. In summary, the results of this study indicated that nine breeds in the Chuan-Yu area were divided into two groups according to geographical distribution. Our study may provide guidance for future research investigating marker-assisted selection and disease-resistant breeding using the SLC11A1 gene.