Current technics for visualizing RNA in a cell
- Авторы: Ibragimov A.1, Kozlov E.1, Kurbidaeva A.1, Ryabichko S.1, Shidlovskii Y.1,2
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Учреждения:
- Institute of Gene Biology
- Department of Biology and General Genetics
- Выпуск: Том 53, № 10 (2017)
- Страницы: 1080-1090
- Раздел: Reviews and Theoretical Articles
- URL: https://journals.rcsi.science/1022-7954/article/view/188468
- DOI: https://doi.org/10.1134/S1022795417100040
- ID: 188468
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Аннотация
The study of RNA functions in a cell is based on the methods of labeling and detection of these molecules. In recent years, considerable progress has been made in this area, making it possible to detect RNA in amounts to one molecule with high specificity and to track the dynamics of spatial RNA distribution in the living cell. The review presents the latest developments in the field of in situ hybridization and the use of RNA aptamers and RNA-binding proteins. The prospects of using the CRISPR-Cas system for RNA visualization are discussed in detail.
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Об авторах
A. Ibragimov
Institute of Gene Biology
Email: yul.biogen@gmail.com
Россия, Moscow, 119334
E. Kozlov
Institute of Gene Biology
Email: yul.biogen@gmail.com
Россия, Moscow, 119334
A. Kurbidaeva
Institute of Gene Biology
Email: yul.biogen@gmail.com
Россия, Moscow, 119334
S. Ryabichko
Institute of Gene Biology
Email: yul.biogen@gmail.com
Россия, Moscow, 119334
Yu. Shidlovskii
Institute of Gene Biology; Department of Biology and General Genetics
Автор, ответственный за переписку.
Email: yul.biogen@gmail.com
Россия, Moscow, 119334; Moscow, 119048