Importance of DNA methylation in the inheritance of radiation-induced aberrant expression of microRNA
- Авторлар: Tarasov V.1, Makhotkin M.1, Boyko N.1, Shin E.1, Tyutyakina M.1, Chikunov I.1, Naboka A.1, Mashkarina A.1, Kirpiy A.2, Matishov D.1
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Мекемелер:
- Institute of Arid Zones, Southern Research Center
- InterLabService Co., Ltd.
- Шығарылым: Том 53, № 5 (2017)
- Беттер: 551-560
- Бөлім: General Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188250
- DOI: https://doi.org/10.1134/S1022795417050118
- ID: 188250
Дәйексөз келтіру
Аннотация
We studied microRNA gene expression in HeLa cells following exposure for 6 h and 8 days to Co60 gamma rays at a dose of 4 Gy using an approach of large-scale parallel DNA sequencing. We identified 12 microRNAs with aberrant expression which were maintained in cell generations. The analysis of radiation-induced aberrant expression of pre-microRNAs made it possible to assess the importance of nuclear and cytoplasmic stages of microRNA biogenesis for preservation of its aberrant expression. On cell treatment by 5-azacytidine, aberrant expression was maintained only in two microRNAs: miR-21-3p and miR-422a, which demonstrated an increase in expression. Radiation-induced decrease in expression in ten examined microRNAs was dependent on DNA demethylation. At the same time, expression in a microRNA set, which demonstrated inheritable alteration of the expression after gamma-radiation exposure in the untreated cells, was not dependent or was weakly dependent on DNA methylation. The obtained results suggest that ionizing radiation induces aberrant DNA methylation, which affects inherited expression changes in microRNAs in cell generations after exposure to the mutagen.
Негізгі сөздер
Авторлар туралы
V. Tarasov
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
M. Makhotkin
Institute of Arid Zones, Southern Research Center
Хат алмасуға жауапты Автор.
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
N. Boyko
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
E. Shin
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
M. Tyutyakina
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
I. Chikunov
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
A. Naboka
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
A. Mashkarina
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006
A. Kirpiy
InterLabService Co., Ltd.
Email: mmakhotkin@mail.ru
Ресей, Moscow, 115035
D. Matishov
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Ресей, Rostov-on-Don, 344006