Importance of DNA methylation in the inheritance of radiation-induced aberrant expression of microRNA
- Autores: Tarasov V.1, Makhotkin M.1, Boyko N.1, Shin E.1, Tyutyakina M.1, Chikunov I.1, Naboka A.1, Mashkarina A.1, Kirpiy A.2, Matishov D.1
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Afiliações:
- Institute of Arid Zones, Southern Research Center
- InterLabService Co., Ltd.
- Edição: Volume 53, Nº 5 (2017)
- Páginas: 551-560
- Seção: General Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188250
- DOI: https://doi.org/10.1134/S1022795417050118
- ID: 188250
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Resumo
We studied microRNA gene expression in HeLa cells following exposure for 6 h and 8 days to Co60 gamma rays at a dose of 4 Gy using an approach of large-scale parallel DNA sequencing. We identified 12 microRNAs with aberrant expression which were maintained in cell generations. The analysis of radiation-induced aberrant expression of pre-microRNAs made it possible to assess the importance of nuclear and cytoplasmic stages of microRNA biogenesis for preservation of its aberrant expression. On cell treatment by 5-azacytidine, aberrant expression was maintained only in two microRNAs: miR-21-3p and miR-422a, which demonstrated an increase in expression. Radiation-induced decrease in expression in ten examined microRNAs was dependent on DNA demethylation. At the same time, expression in a microRNA set, which demonstrated inheritable alteration of the expression after gamma-radiation exposure in the untreated cells, was not dependent or was weakly dependent on DNA methylation. The obtained results suggest that ionizing radiation induces aberrant DNA methylation, which affects inherited expression changes in microRNAs in cell generations after exposure to the mutagen.
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Sobre autores
V. Tarasov
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
M. Makhotkin
Institute of Arid Zones, Southern Research Center
Autor responsável pela correspondência
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
N. Boyko
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
E. Shin
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
M. Tyutyakina
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
I. Chikunov
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
A. Naboka
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
A. Mashkarina
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006
A. Kirpiy
InterLabService Co., Ltd.
Email: mmakhotkin@mail.ru
Rússia, Moscow, 115035
D. Matishov
Institute of Arid Zones, Southern Research Center
Email: mmakhotkin@mail.ru
Rússia, Rostov-on-Don, 344006