The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli
- Authors: Smirnova S.V.1, Abilev S.K.1,2, Igonina E.V.1, Glaser V.M.2, Parmon V.N.3, Yankovsky N.K.1,2
-
Affiliations:
- Vavilov Institute of General Genetics
- Department of Genetics
- Boreskov Institute of Catalysis, Siberian Branch
- Issue: Vol 54, No 8 (2018)
- Pages: 919-924
- Section: General Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/189102
- DOI: https://doi.org/10.1134/S1022795418080124
- ID: 189102
Cite item
Abstract
This study has shown for the first time that deuterium oxide (D2O) enhances expression of the E. coli ada-regulon induced by alkylating compounds, N-nitroso-N-methylurea (NMU) and methyl methanesulfonate (MMS). To compare the induction of ada-regulon in deuterated and in non-deuterated (control) cultures of E. coli, a biosensor based on the E. coli strain K12 MG1655 (pAlkA–lux) luminescing as a result of activating the alkA gene promoter in response to DNA alkylation was used. Depending on the D2O concentration from 5 to 10% in the pre-deuterium medium, these alkylating compounds at a concentration of 0.005 M induced expression of the alkA gene from 1.3 to 5 times higher as compared to induction in nondeuterated culture. It is suggested that deuterium can be one of the main factors in stabilizing the bond between the promoter and the alkylated Ada protein, leading to increased transcription of the ada-regulon.
About the authors
S. V. Smirnova
Vavilov Institute of General Genetics
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991
S. K. Abilev
Vavilov Institute of General Genetics; Department of Genetics
Author for correspondence.
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991; Moscow, 119991
E. V. Igonina
Vavilov Institute of General Genetics
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991
V. M. Glaser
Department of Genetics
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991
V. N. Parmon
Boreskov Institute of Catalysis, Siberian Branch
Email: abilev@vigg.ru
Russian Federation, Novosibirsk, 630090
N. K. Yankovsky
Vavilov Institute of General Genetics; Department of Genetics
Email: abilev@vigg.ru
Russian Federation, Moscow, 119991; Moscow, 119991