The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

This study has shown for the first time that deuterium oxide (D2O) enhances expression of the E. coli ada-regulon induced by alkylating compounds, N-nitroso-N-methylurea (NMU) and methyl methanesulfonate (MMS). To compare the induction of ada-regulon in deuterated and in non-deuterated (control) cultures of E. coli, a biosensor based on the E. coli strain K12 MG1655 (pAlkA–lux) luminescing as a result of activating the alkA gene promoter in response to DNA alkylation was used. Depending on the D2O concentration from 5 to 10% in the pre-deuterium medium, these alkylating compounds at a concentration of 0.005 M induced expression of the alkA gene from 1.3 to 5 times higher as compared to induction in nondeuterated culture. It is suggested that deuterium can be one of the main factors in stabilizing the bond between the promoter and the alkylated Ada protein, leading to increased transcription of the ada-regulon.

Авторлар туралы

S. Smirnova

Vavilov Institute of General Genetics

Email: abilev@vigg.ru
Ресей, Moscow, 119991

S. Abilev

Vavilov Institute of General Genetics; Department of Genetics

Хат алмасуға жауапты Автор.
Email: abilev@vigg.ru
Ресей, Moscow, 119991; Moscow, 119991

E. Igonina

Vavilov Institute of General Genetics

Email: abilev@vigg.ru
Ресей, Moscow, 119991

V. Glaser

Department of Genetics

Email: abilev@vigg.ru
Ресей, Moscow, 119991

V. Parmon

Boreskov Institute of Catalysis, Siberian Branch

Email: abilev@vigg.ru
Ресей, Novosibirsk, 630090

N. Yankovsky

Vavilov Institute of General Genetics; Department of Genetics

Email: abilev@vigg.ru
Ресей, Moscow, 119991; Moscow, 119991

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML

© Pleiades Publishing, Inc., 2018