Species Identification of Multimammate Rats of the Genus Mastomys (Rodentia: Muridae) in Eastern Africa Using PCR Typing of Cytochrome b Gene Fragments

Abstract

The multimammate rats of the genus Mastomys are widespread throughout sub-Saharan Africa. They are the major agricultural pests and reservoirs of many infections dangerous to humans. A simple and accurate species identification of multimammate rats is crucial in ecological and epidemiological studies; however, it is complicated by the absence of pronounced morphological differentiation between Mastomys species. We describe a simple molecular assay based on PCR typing of the cytochrome b gene fragments as a method that allows fast genotyping of a large number of samples without sequencing to distinguish Mastomys species of East Africa.