Species Identification of Multimammate Rats of the Genus Mastomys (Rodentia: Muridae) in Eastern Africa Using PCR Typing of Cytochrome b Gene Fragments
- Авторы: Martynov A.1, Lavrenchenko L.1
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Учреждения:
- Severtsov Institute of Ecology and Evolution
- Выпуск: Том 54, № 7 (2018)
- Страницы: 874-877
- Раздел: Short Communications
- URL: https://journals.rcsi.science/1022-7954/article/view/189085
- DOI: https://doi.org/10.1134/S1022795418070104
- ID: 189085
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Аннотация
The multimammate rats of the genus Mastomys are widespread throughout sub-Saharan Africa. They are the major agricultural pests and reservoirs of many infections dangerous to humans. A simple and accurate species identification of multimammate rats is crucial in ecological and epidemiological studies; however, it is complicated by the absence of pronounced morphological differentiation between Mastomys species. We describe a simple molecular assay based on PCR typing of the cytochrome b gene fragments as a method that allows fast genotyping of a large number of samples without sequencing to distinguish Mastomys species of East Africa.
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Об авторах
A. Martynov
Severtsov Institute of Ecology and Evolution
Автор, ответственный за переписку.
Email: aleksey.martynov@mail.ru
Россия, Moscow, 119071
L. Lavrenchenko
Severtsov Institute of Ecology and Evolution
Email: aleksey.martynov@mail.ru
Россия, Moscow, 119071