Induction of transcription through the scs insulator leads to abnormal development of Drosophila melanogaster
- Authors: Kyrchanova O.V.1, Leman D.V.1, Toshchakov S.V.1, Utkina M.V.1, Tikhonov M.V.1, Parshikov A.F.1, Maksimenko O.G.1, Georgiev P.G.1
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Affiliations:
- Institute of Gene Biology
- Issue: Vol 52, No 10 (2016)
- Pages: 1007-1014
- Section: Molecular Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/187910
- DOI: https://doi.org/10.1134/S1022795416100057
- ID: 187910
Cite item
Abstract
A regulatory element named scs is one of the first insulators discovered in Drosophila, which was found on the boundary of the hsp70 domain. The 993-bp scs insulator contains two promoters at the ends and two polyadenylation signals located in the same orientation in the central part of the insulator. In the Drosophila transgenic lines, induction of a strong transcription through the scs insulator only in the direction that coincides with the direction of the two polyadenylation sites activity results in multiple phenotypic defects of the Drosophila development and embryonic lethality. A similar effect was not observed upon testing of other known Drosophila insulators.
Keywords
About the authors
O. V. Kyrchanova
Institute of Gene Biology
Author for correspondence.
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334
D. V. Leman
Institute of Gene Biology
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334
S. V. Toshchakov
Institute of Gene Biology
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334
M. V. Utkina
Institute of Gene Biology
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334
M. V. Tikhonov
Institute of Gene Biology
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334
A. F. Parshikov
Institute of Gene Biology
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334
O. G. Maksimenko
Institute of Gene Biology
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334
P. G. Georgiev
Institute of Gene Biology
Email: olgina73@gmail.com
Russian Federation, Moscow, 119334