Identification of cadmium-responsive microRNAs in Solanum torvum by high-throughput sequencing


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Resumo

MicroRNAs (miRNAs) play key roles in regulating Cd toxicity tolerance in plants. Solanum torvum Sw. is a typical low Cd-accumulating plant that has a high Cd tolerance. Despite its importance, no miRNA has been identified from S. torvum thus far. In this study, high-throughput sequencing of S. torvum small RNAs was used to identify miRNAs that were differentially expressed in response to Cd toxicity. At least 45 miRNA families and 165 individual members within those families were identified in both the control and Cd-treated S. torvum roots. Among these miRNAs, 45 miRNAs from 21 miRNA families were differentially expressed in the control and Cd-treated S. torvum plants. Among these 21 differentially expressed miRNA families, 6 miRNA families were upregulated in the Cd-treated roots, and 15 miRNA families were downregulated in Cd-treated roots. Bioinformatics analysis indicated that these miRNAs were involved in replication, recombination and repair, inorganic ion transport and metabolism, transcription, signal transduction mechanisms, cell cycle control, cell division, chromosome partitioning, RNA processing and modification in S. torvum roots. These results indicated that specific miRNAs are tightly regulated by Cd toxicity in the roots of S. torvum, which may play key roles in the Cd tolerance of S. torvum.

Sobre autores

X. Kang

College of Horticulture

Email: xujin@xtbg.ac.cn
República Popular da China, Taigu, Shanxi, 030801

J. Gao

College of Horticulture; Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden

Email: xujin@xtbg.ac.cn
República Popular da China, Taigu, Shanxi, 030801; Menglun, Mengla, Yunnan, 666303

J. Zhao

Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden

Email: xujin@xtbg.ac.cn
República Popular da China, Menglun, Mengla, Yunnan, 666303

H. Yin

Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Gansu Province, Department of Ecology and Agriculture Research, Cold and Arid Regions Environmental and Engineering Research Institute

Email: xujin@xtbg.ac.cn
República Popular da China, Lanzhou, Gansu, 730000

W. Wang

College of Life Science and Geography

Email: xujin@xtbg.ac.cn
República Popular da China, Xining, 810008

P. Zhang

Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden

Email: xujin@xtbg.ac.cn
República Popular da China, Menglun, Mengla, Yunnan, 666303

R. Wang

Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden

Email: xujin@xtbg.ac.cn
República Popular da China, Menglun, Mengla, Yunnan, 666303

J. Xu

Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden

Autor responsável pela correspondência
Email: xujin@xtbg.ac.cn
República Popular da China, Menglun, Mengla, Yunnan, 666303

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