Involvement of ethylene in UV-B-induced changes in polyamine content in Arabidopsis thaliana plants
- Authors: Prudnikova O.N.1, Rakitina T.Y.1, Karyagin V.V.1, Vlasov P.V.1, Rakitin V.Y.1
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Affiliations:
- Timiryazev Institute of Plant Physiology
- Issue: Vol 63, No 5 (2016)
- Pages: 604-608
- Section: Research Papers
- URL: https://journals.rcsi.science/1021-4437/article/view/179273
- DOI: https://doi.org/10.1134/S1021443716050101
- ID: 179273
Cite item
Abstract
Components of the ethylene signal perception and transduction pathway (ethylene signaling pathway, ESP) were studied in respect to their involvement in regulation of UV-B-induced changes in levels of polyamines in plants Arabidopsis thaliana (L.) Heynh. Experiments were performed on 15-day old wild type (WT) plants, the mutant etr1-1 with impaired ethylene reception, and the ethylene-insensitive mutant ctr1-1 with constitutively activated ESP. The plants were cultivated aseptically. It was found that exogenous ethylene or an inhibitor of its action 1-methylcyclopropen (1-MCP), which blocks ethylene receptors did not affect the polyamine content in leaf rosettes of plants, which had not been subjected to UV-B stress. A day after UV-B irradiation at intermediate (9 kJ/m2) or high doses (18 kJ/m2), the putrescine levels increased, respectively, 6.4 and 3.0 times in WT, 4.5 and 3.2 times in etr1-1, and 5.5 and 4.7 in ctr1-1. Pretreatment with ethylene (1 μL/L) for 24 h reduced the putrescine accumulation along with the loss in spermidine and spermine pools in WT plants and, to a lesser extent, in etr1-1 mutant. Treatment with 1-MCP (50 nL/L, 3 h before and 24 h after the irradiation) enhanced plant sensitivity to UV-B, putrescine accumulation, as well as spermidine and spermine consumption in WT and, to a lesser degree, in etr1-1. The mutant ctr1-1 was insensitive to both ethylene and 1-MCP. The results show that the activation of ESP by ethylene increases plant resistance to UV-B because the irradiation stimulates accumulation of putrescine, which converts to spermidine and spermine functioning as ROS traps.
About the authors
O. N. Prudnikova
Timiryazev Institute of Plant Physiology
Email: rakit@ippras.ru
Russian Federation, ul. Botanicheskaya 35, Moscow, 127276
T. Ya. Rakitina
Timiryazev Institute of Plant Physiology
Email: rakit@ippras.ru
Russian Federation, ul. Botanicheskaya 35, Moscow, 127276
V. V. Karyagin
Timiryazev Institute of Plant Physiology
Email: rakit@ippras.ru
Russian Federation, ul. Botanicheskaya 35, Moscow, 127276
P. V. Vlasov
Timiryazev Institute of Plant Physiology
Email: rakit@ippras.ru
Russian Federation, ul. Botanicheskaya 35, Moscow, 127276
V. Yu. Rakitin
Timiryazev Institute of Plant Physiology
Author for correspondence.
Email: rakit@ippras.ru
Russian Federation, ul. Botanicheskaya 35, Moscow, 127276