Dependence of root biomass accumulation on the content and metabolism of cytokinins in ethylene-insensitive plants
- Authors: Korobova A.V.1, Vysotskaya L.B.1, Vasinskaya A.N.1, Kuluev B.R.2, Veselov S.Y.3, Kudoyarova G.R.1
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Affiliations:
- Ufa Institute of Biology
- Institute of Biochemistry and Genetics, Ufa Scientific Center
- Bashkir State University
- Issue: Vol 63, No 5 (2016)
- Pages: 597-603
- Section: Research Papers
- URL: https://journals.rcsi.science/1021-4437/article/view/179270
- DOI: https://doi.org/10.1134/S1021443716050071
- ID: 179270
Cite item
Abstract
Diverse functions of ethylene in plants may depend on its ability to interact with other hormones. We studied the participation of ethylene in the regulation of accumulation and metabolism of cytokinins comparing ethylene-insensitive mutant plants of arabidopsis (Arabidopsis thaliana [L.] Heynh., etr1-1) with the plants of original ecotype Columbia (Col-0). Because cytokinins can regulate growth of both leaves and roots, we determined the weights of these organs and the ratio between them. The content of zeatin and its riboside in the roots of etr1-1 plants was two times greater than in Col-0 plants, which could be accounted for by inhibition of conversion of these forms of cytokinins into 9-N-glucosides. In the leaves of mutant plants, expression of IPT3 gene responsible for the synthesis of cytokinins was more intense than in Col-0 plants, which could also contribute to a rise in the content of cytokinins. In this case, the weight of roots in etr1-1 mutants was lower than in the plants of original ecotype. Because high concentrations of cytokinins can inhibit root growth, suppression of accumulation of their biomass in mutant plants may be related to a greater content of cytokinins therein. The obtained results suggest that ethylene can suppress accumulation of cytokinins and, thereby, maintain redistribution of biomass in favor of the roots, which is important for plant adaptation to a shortage of water and ions.
Keywords
About the authors
A. V. Korobova
Ufa Institute of Biology
Email: guzel@anrb.ru
Russian Federation, pr. Oktyabrya 69, Ufa, 450054
L. B. Vysotskaya
Ufa Institute of Biology
Email: guzel@anrb.ru
Russian Federation, pr. Oktyabrya 69, Ufa, 450054
A. N. Vasinskaya
Ufa Institute of Biology
Email: guzel@anrb.ru
Russian Federation, pr. Oktyabrya 69, Ufa, 450054
B. R. Kuluev
Institute of Biochemistry and Genetics, Ufa Scientific Center
Email: guzel@anrb.ru
Russian Federation, pr. Oktyabrya 71, Ufa, 450054
S. Yu. Veselov
Bashkir State University
Email: guzel@anrb.ru
Russian Federation, ul. Kirova 65, Ufa, 450074
G. R. Kudoyarova
Ufa Institute of Biology
Author for correspondence.
Email: guzel@anrb.ru
Russian Federation, pr. Oktyabrya 69, Ufa, 450054