The Effect of Shape and Concentration on Translational Diffusion of Proteins Measured by PFG NMR
- Authors: Kusova A.M.1,2, Sitnitsky A.E.1, Idiyatullin B.Z.1, Bakirova D.R.1, Zuev Y.F.1,2,3
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Affiliations:
- Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences
- Kazan (Volga Region) Federal University
- Kazan State Power Engineering University
- Issue: Vol 49, No 1 (2018)
- Pages: 35-51
- Section: Original Paper
- URL: https://journals.rcsi.science/0937-9347/article/view/247998
- DOI: https://doi.org/10.1007/s00723-017-0957-y
- ID: 247998
Cite item
Abstract
The concentration dependences of self-diffusion coefficient for irregular-shaped fibrinogen, for globular, spheroidal trypsin and α-chymotrypsin were studied by pulsed field gradient nuclear magnetic resonance. The experimental data were analyzed in a view of two known theoretical approaches—the hydrodynamic model of rigid spheres by Tokuyama and Oppenheim and the phenomenological approach based on the frictional formalism of non-equilibrium thermodynamics by Vink. The detailed discussion of their merits and drawbacks is presented. Our results testify that the Vink’s approach is quite universal, providing a satisfactory description of experimental data for proteins of complicated structure and different shape while the model of Tokuyama and Oppenheim is applicable only to proteins of more regular shape.
About the authors
A. M. Kusova
Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences; Kazan (Volga Region) Federal University
Email: yufzuev@mail.ru
Russian Federation, Lobachevsky Str, 2/31, Kazan; Kremlevskaya Str., 18, Kazan, 420008
A. E. Sitnitsky
Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences
Email: yufzuev@mail.ru
Russian Federation, Lobachevsky Str, 2/31, Kazan
B. Z. Idiyatullin
Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences
Email: yufzuev@mail.ru
Russian Federation, Lobachevsky Str, 2/31, Kazan
D. R. Bakirova
Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences
Email: yufzuev@mail.ru
Russian Federation, Lobachevsky Str, 2/31, Kazan
Yu. F. Zuev
Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences; Kazan (Volga Region) Federal University; Kazan State Power Engineering University
Author for correspondence.
Email: yufzuev@mail.ru
ORCID iD: 0000-0002-6715-2530
Russian Federation, Lobachevsky Str, 2/31, Kazan; Kremlevskaya Str., 18, Kazan, 420008; Krasnoselskaya Str., 51, Kazan, 420066