Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of Streptococcus agalactiae. Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions of group B Streptococcus (GBS) surface antigen ScaAB gene parts of different length in-frame between signal peptide and HA1 were constructed by means of reverse genetics. Viral vector vaccine H7-ScaAB-85 expressing the shortest (85 amino acids) polypeptide from lipoprotein ScaAB linked to HA via a flexible linker was genetically stable, replicated in embryonated chicken eggs and MDCK cells at the same level as cold-adapted influenza A (H7N9) strain. An increase of ScaAB polypeptide insertion length up to 141 amino acids resulted in a decrease of the viral replication level in both susceptible systems and intense mutagenesis of hybrid HA-gene of recombinant influenza A virus in the gene region encoding foreign bacterial lipoprotein. These findings suggest that H7-ScaAB-85 is a promising vaccine candidate, which should be further assessed using a mouse model.

作者简介

T. Smolonogina

Institute of Experimental Medicine

编辑信件的主要联系方式.
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

I. Isakova-Sivak

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

T. Kotomina

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

A. Evsina

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

E. Stepanova

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

P. Prokopenko

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

G. Leontieva

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

A. Suvorov

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

L. Rudenko

Institute of Experimental Medicine

Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376

补充文件

附件文件
动作
1. JATS XML

版权所有 © Allerton Press, Inc., 2019