Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus
- 作者: Smolonogina T.A.1, Isakova-Sivak I.N.1, Kotomina T.S.1, Evsina A.S.1, Stepanova E.A.1, Prokopenko P.I.1, Leontieva G.F.1, Suvorov A.N.1, Rudenko L.G.1
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隶属关系:
- Institute of Experimental Medicine
- 期: 卷 34, 编号 1 (2019)
- 页面: 25-34
- 栏目: Experimental Works
- URL: https://journals.rcsi.science/0891-4168/article/view/178332
- DOI: https://doi.org/10.3103/S0891416819010087
- ID: 178332
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详细
The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of Streptococcus agalactiae. Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions of group B Streptococcus (GBS) surface antigen ScaAB gene parts of different length in-frame between signal peptide and HA1 were constructed by means of reverse genetics. Viral vector vaccine H7-ScaAB-85 expressing the shortest (85 amino acids) polypeptide from lipoprotein ScaAB linked to HA via a flexible linker was genetically stable, replicated in embryonated chicken eggs and MDCK cells at the same level as cold-adapted influenza A (H7N9) strain. An increase of ScaAB polypeptide insertion length up to 141 amino acids resulted in a decrease of the viral replication level in both susceptible systems and intense mutagenesis of hybrid HA-gene of recombinant influenza A virus in the gene region encoding foreign bacterial lipoprotein. These findings suggest that H7-ScaAB-85 is a promising vaccine candidate, which should be further assessed using a mouse model.
作者简介
T. Smolonogina
Institute of Experimental Medicine
编辑信件的主要联系方式.
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
I. Isakova-Sivak
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
T. Kotomina
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
A. Evsina
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
E. Stepanova
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
P. Prokopenko
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
G. Leontieva
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
A. Suvorov
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
L. Rudenko
Institute of Experimental Medicine
Email: smolonogina@mail.ru
俄罗斯联邦, St. Petersburg, 197376
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