Express Screening of Reassortants of Influenza Virus B—Candidates for Vaccinating Strains of Live Influenza Vaccine by Using Pyrosequencing

E. V. Krutikova; E. A. Stepanova; I. V. Kiseleva; L. G. Rudenko

doi:10.3103/S089141681802009X

Express Screening of Reassortants of Influenza Virus B—Candidates for Vaccinating Strains of Live Influenza Vaccine by Using Pyrosequencing

作者: Krutikova E.V.¹, Stepanova E.A.¹, Kiseleva I.V.¹, Rudenko L.G.¹
隶属关系:
1. Institute of Experimental Medicine
期: 卷 33, 编号 2 (2018)
页面: 139-144
栏目: Experimental Works
URL: https://journals.rcsi.science/0891-4168/article/view/178286
DOI: https://doi.org/10.3103/S089141681802009X
ID: 178286

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For the rapid preparation of strains of live influenza vaccine, it is important to have a rapid method for screening vaccine reassortants. In this paper, a method of pyrosequencing was used to analyze the genome of reassortants obtained by crossing the donor of attenuation B/Leningrad/14/17/55 and the wild-type virus B/Indiana/25/2015. It has been shown that pyrosequencing allows the composition of the reassortant genome to be evaluated quickly, economically, and with high accuracy.