Quantitative Analysis of the Microbiota of Periodontal Pockets and Saliva by Real-Time PCR before and after Treatment of Periodontitis

Resumo

Work has been done on standardization of detection and quantification of periodontopathogenic microorganisms in the clinical material (contents of periodontal pockets and saliva) using real-time PCR. To optimize the conditions for the analysis, a method for obtaining clinical samples of known volume was developed and a calibration sample to obtain reliable results in the diagnosis of periodontitis was designed.