Email this article Bacteriophages of soil bacilli: A new multivalent phage of Bacillus altitudinis
- Authors: Shah Mahmud R.1, Garifulina K.I.1, Ulyanova V.V.1, Evtugyn V.G.1, Mindubaeva L.N.1, Khazieva L.R.1, Dudkina E.V.1, Vershinina V.I.1, Kolpakov A.I.1, Ilinskaya O.N.1
-
Affiliations:
- Institute of Fundamental Medicine and Biology
- Issue: Vol 32, No 2 (2017)
- Pages: 87-93
- Section: Experimental Works
- URL: https://journals.rcsi.science/0891-4168/article/view/178189
- DOI: https://doi.org/10.3103/S0891416817020082
- ID: 178189
Cite item
Open Access
Access granted
Subscription Access
Abstract
Bacillus are soil saprophytes, facultative anaerobes developing in the temperature range of 28–37°С. 16S rRNA cataloging shows that these bacteria form a coherent class with broad variability of virulence. Bacillus phages can be extensively used for phagotyping bacteria in the process of soil, water, and food monitoring. Bacillus phages can also be used as vectors in horizontal gene transfer and potential therapeutic agents. Thus, description of the biological diversity of the Bacillus phages is useful for further development of tools used in molecular biology and biomedicine. In this work, the scheme for isolation of soil bacteriophages was unified, which allowed ten bacillus phages to be isolated from different types of soil. It was shown that the number of phages depended on the soil fertility, decreasing as the soil changed from black soil to chestnut soil to gray forest soil to uncontaminated urban soil to oil-contaminated urban soil. A new polyvalent DNA-containing bacteriophage SRT01hs of B. altitudinis (it is also able to infect B. subtilis, B. cereus, and B. pumilus, but not B. licheniformis and B. atrophaeus) was described in detail. It has a typical structure: a total length of 360 nm and an icosahedron-shaped head 100 nm in diameter. Several phages simultaneously attack a B. altitudinis cell by increasing the level of intracellular low-molecular RNA. Infection with the phage virtually eliminates the stationary growth phase of infected bacilli and leads to a permanent increase in the number of phages in cultural liquor, with the exception of the time period of high activity of the secreted ribonuclease.
About the authors
R. Shah Mahmud
Institute of Fundamental Medicine and Biology
Author for correspondence.
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
K. I. Garifulina
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
V. V. Ulyanova
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
V. G. Evtugyn
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
L. N. Mindubaeva
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
L. R. Khazieva
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
E. V. Dudkina
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
V. I. Vershinina
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
A. I. Kolpakov
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
O. N. Ilinskaya
Institute of Fundamental Medicine and Biology
Email: raihan.shah@gmail.com
Russian Federation, Kazan, 420008
Supplementary files
