A new genetic variant of the Crimean–Congo hemorrhagic fever virus isolated in Crimea

A. N. Kulichenko; A. S. Volynkina; E. S. Kotenev; S. V. Pisarenko; L. I. Shaposhnikova; Y. V. Lisitskaya; N. F. Vasilenko; O. I. Tsygankova; Y. M. Evchenko; Y. M. Tohov; V. N. Savel’ev; S. N. Tihonov; N. A. Penkovskaya

doi:10.3103/S0891416816020051

A new genetic variant of the Crimean–Congo hemorrhagic fever virus isolated in Crimea

Authors: Kulichenko A.N.¹, Volynkina A.S.¹, Kotenev E.S.¹, Pisarenko S.V.¹, Shaposhnikova L.I.¹, Lisitskaya Y.V.¹, Vasilenko N.F.¹, Tsygankova O.I.¹, Evchenko Y.M.¹, Tohov Y.M.¹, Savel’ev V.N.¹, Tihonov S.N.², Penkovskaya N.A.³
Affiliations:
1. Stavropol Research Antiplague Institute
2. Crimea Republic Plague Control Station
3. Federal Service on Customers’ Rights Protection and Human Wellbeing Surveillance Interregional Directorate in the Crimea Republic and Sevastopol Federal City
Issue: Vol 31, No 2 (2016)
Pages: 94-101
Section: Experimental Works
URL: https://journals.rcsi.science/0891-4168/article/view/178068
DOI: https://doi.org/10.3103/S0891416816020051
ID: 178068

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Statistics

Abstract

The work presents the results of genetic identification of Crimean–Congo hemorrhagic fever (CCHF) virus isolates obtained in the Crimean federal district in the course of the 2015 epidemiological survey for a Crimean-Congo hemorrhagic fever case from Crimea. A PCR test for the presence of the CCHF virus RNA was performed for a single blood serum sample obtained from a patient and for 61 pools (506 individuals) of ixodic ticks collected during an epizootological study carried out in six administrative regions of the Crimean federal district. CCHF virus RNA was detected in the patient’s blood serum and in ten samples of ixodic ticks. Genetic identification of the CCHF virus isolates was performed by sequencing S, M, and L virus genome segments. The results of the molecular and genetic analysis showed that the RNAs of the CCHF viruses detected in the blood serum samples and three tick suspension samples are highly homologous to each other and belong to a new Crimea (Vd) genetic subgroup of the Europe 1 genotype. Whole genome sequences were obtained for two CCHF virus isolates from the Crimea subgroup (Vd). CCHF virus variants belonging to the Crimea subgroup (Vd) of the Europe 1 genotype have been described for the first time and are endemic for the Crimean peninsula.

Keywords

Crimean–Congo hemorrhagic fever, molecular epidemiology, whole genome sequencing, phylogenetic analysis, Crimean federal district

Username
Password
Remember me

Forgot password?	Register

Username
Password
Remember me

Forgot password?	Register

A new genetic variant of the Crimean–Congo hemorrhagic fever virus isolated in Crimea

Full Text

Abstract

Keywords

About the authors

This website uses cookies