Involvement of Dihydropyrimidinase-Related Protein 2 in Regulation of Anxiety in Humans

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The goal of the study was analysis of the role for dihydropyrimidinase-related protein 2 (DRP2) in regulation of anxiety in humans and animals. For this purpose, the levels of DRP2 in the platelets and saliva and the levels of natural autoantibodies to DRP2 in the blood serum of the patients, admitted for a surgical operation, were analyzed alongside with the effects of DRP2 on rat behavior using the model of elevated plus-maze. Blood samples were collected from the veins of the patients, feeling anxiety at the day of surgery, into test tubes containing 5% EDTA as an anticoagulant. Platelets and serum were purified and through indirect ELISA-test the levels of DRP2 were evaluated in the platelets, while the levels of natural anti-DRP2 autoantibodies were measured in the blood serum. Concomitantly, the levels of DRP2 were estimated in the patients’ saliva sampled on the same day. The results showed upregulation of DRP2 in the platelets (p < 0.05 on Student’s t-criterion) and natural anti-DRP2 autoantibodies in the serum of the patients (p < 0.001) relative to healthy persons. Evaluation of the level of the stress hormone cortisol in the patients’ serum revealed its upregulation (p < 0.01). At the same time downregulation of DRP2 in the patients’ saliva (p < 0.01) was noticed. Intra-cerebral administration of DRP2 to the rats resulted in an increase in the number of crossed squares in the open arms of the maze relative to the control rats injected with inactivated DRP2 (p < 0.01, Wilcoxon and Mann–Whitney U-criterion) which indicates their reduced anxiety. Based on the data obtained we conclude that evaluation of DRP2 in the platelets, natural anti-DRP2 autoantibodies in the blood serum and DRP2 in the saliva might serve as an indicator of anxiety levels in humans.

Sobre autores

Sh. Guliyeva

Nakhchivan State University

Email: arifmekht@yahoo.com
Azerbaijan, Nakhchivan

A. Mekhtiev

Academician Abdulla Garayev Institute of Physiology, NAS

Autor responsável pela correspondência
Email: arifmekht@yahoo.com
Azerbaijan, Baku

Bibliografia

  1. Shapell S, Detwiler C, Boquet A, Wiegmann D (2006) Human error and commercial aviation accidents: A comprehensive, fine-grained analysis using HFACS. Federal Aviation Administration. DOT/FAA/AM-06/18. Office of Aerospace Medicine Washington, DC 20591.
  2. Devido G, Jones M, Geraci M, Hollon N, Blair RJ, Pine DS, Blair K (2009) Stimulus-reinforcement-based decision making and anxiety: impairment in generalized anxiety disorder (GAD) but not in generalized social phobia (GSP). Psychol Med 39: 1153–1161. https://doi.org/10.1017/S003329170800487X
  3. Luhman CC, Ishida K, Hajcak G (2011) Intolerance of uncertainty and decisions about delayed, probabilistic rewards. Behav Ther 42: 378–386. https://doi.org/10.1016/j.beth.2010.09.002
  4. Teng C, Otero M, Geraci M, Blair RJR, Pine DS, Grillon Ch, Blair KS (2016) Abnormal decision-making in generalized anxiety disorder: Aversion of risk or stimulus-reinforcement impairment? Psych Res 237: 351–356. https://doi.org/10.1016/j.psychres.2015.12.031
  5. Elliott JM, Kent A (1989) Comparison of [125I]iodolysergic acid diethylamide binding to human frontal cortex and platelet tissue. J Neurochem 53: 191–196. https://doi.org/10.1111/j.1471-4159.1989.tb07313.x
  6. Collins ChM, Kloek J, Elliott JM (2013) Parallel changes in serotonin levels in brain and blood following acute administration of MDMA. J Psychopharm 27: 109–112. https://doi.org/10.1177/0269881112463123
  7. Мехтиев АА (2000) Обнаружение в головном мозге крыс белка, обладающего антиконсолидационными свойствами. Бюл экспер биол мед 129: 147–150. [Mekhtiev AA (2000) Revealing in the brain of rats the protein possessing anticonsolidation properties. Bull Exp Biol Med 129: 147–150. (In Russ)].https://doi.org/10.1007/BF02766081
  8. Goshima Y, Nakamura F, Strittmatter P, Strittmatter SM (1995) Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33. Nature 376: 509–514. https://doi.org/10.1038/376509a0
  9. Inagaki N, Chihara K, Arimura N, Ménager C, Kawano MN, Matsuo N, Nishimura T, Amano M, Kaibuchi K (2001) CRMP-2 induces axons in cultured hippocampal neurons. Nat Neurosci 4: 781–782. https://doi.org/10.1038/90476
  10. Bisgaard ChF, Jayatissa MN, Enghild JJ, Sanchéz C, Artemychyn R, Wiborg O (2007) Proteomic investigation of the ventral rat hippocampus links DRP-2 to escitalopram treatment resistance and SNAP to stress resilience in the chronic mild stress model of depression. J Mol Neurosci 32: 132–144. https://doi.org/10.1007/s12031-007-0025-4
  11. Wei Y, Wang G, Chen J, Xiao L, Wu Z, He J, Zhang N (2021) Maternal deprivation induces cytoskeletal alterations and depressive-like behavior in adult male rats by regulating the AKT/GSK3β/CRMP2 signaling pathway. Physiol & Behav 242: 113625. https://doi.org/10.1016/j.physbeh.2021.113625
  12. Xiang D, Xiao J, Sun S, Fu L, Yao L, Wang G, Liu Z (2020) Differential regulation of DNA methylation at the CRMP2 promoter region between the hippocampus and prefrontal cortex in a CUMB depression model. Front Psychiatr 11: 141. https://doi.org/10.3389/fpsyt.2020.00141
  13. Walf AA, Frye CA (2007) The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat Protoc 2: 322–328. https://doi.org/10.1038/nprot.2007.44
  14. Kraeuter A-K, Guest PC, Sarnyai Z (2019) The Elevated Plus Maze Test for Measuring Anxiety-Like Behavior in Rodents. Methods Mol Biol 1916: 69–74. https://doi.org/10.1007/978-1-4939-8994-2_4
  15. File SE, Zangrossi H Jr, Sanders FL, Mabbutt PS (1994) Raised corticosterone in the rat after exposure to the elevated plus-maze. Psychopharmacology 113: 543–546. https://doi.org/10.1007/BF02245237
  16. Rodgers RJ, Haller J, Holmes A, Halasz J, Walton TJ, Brain PF (1999) Corticosterone response to the plus-maze: high correlation with risk assessment in rats and mice. Physiol Behav 68: 47–53. https://doi.org/10.1016/s0031-9384(99)00140-7
  17. Hasanova LF (2022) The changes of serotonin-modulating anticonsolidation protein and dihydropyrimidinase-related protein 2 in the amygdala and blood of depressive rats. Azerb J Physiol 37: 7–12.

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Declaração de direitos autorais © Ш.М. Гулиева, А.А. Мехтиев, 2023

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