Morphological adrenal glands changes in rats with different individual-typological behavior features in the PTSD model after dalargin injections

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Abstract

Morphological changes in the adrenal glands of male Wistar rats were studied after intramuscular injection of dalargin (a synthetic analog of leuenkephalin) in a model of post-traumatic stress disorders (PTSD). According to the results of testing in the T-maze and the elevated plus maze (ECL), the rats were divided into groups: active of the low anxiety (ALA), active of the high anxiety (AHA), passive of the low anxiety (PLA), passive of the high anxiety (PHA). In ALA rats after injection of dalargin in the PTSD model the thickness of the fascicular zone (zF) of the adrenal cortex increased by 14%, the area of nuclei in zF cells by 9.5%, and the area of the medulla by 21%. With the development of a PTSD-like state in AHA rats, the thickness of the zF increased by 10%. After injection of dalargin in the PTSD model the thickness of the zF was like control. With the development of a PTSD-like state in PLA rats, the thickness of the zF increased by 17%, and the area of zF cells nuclei, the area of the adrenal medulla and the area nucleis adrenal medulla are decrease by 10.5%, 41% and 8%, respectively. After injection of dalargin in the PTSD model area of the adrenal medullas nuclei decrease more by 7%. In PHA rats after injection of dalargin in the PTSD model the thickness zF increased by 23%. It was concluded that the effect of dalargin on the morphometric parameters of the adrenal glands in modeling PTSD is determined by individual typological features of behavior.

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O. G. Semenova

Pavlov Institute of Physiology of the Russian Academy of Sciences

Author for correspondence.
Email: SemenovaOG@infran.ru
Russian Federation, St. Petersburg

A. V. Vyushina

Pavlov Institute of Physiology of the Russian Academy of Sciences

Email: SemenovaOG@infran.ru
Russian Federation, St. Petersburg

A. V. Pritvorova

Pavlov Institute of Physiology of the Russian Academy of Sciences

Email: SemenovaOG@infran.ru
Russian Federation, St. Petersburg

V. V. Rakitskaya

Pavlov Institute of Physiology of the Russian Academy of Sciences

Email: SemenovaOG@infran.ru
Russian Federation, St. Petersburg

N. E. Ordyan

Pavlov Institute of Physiology of the Russian Academy of Sciences

Email: SemenovaOG@infran.ru
Russian Federation, St. Petersburg

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Supplementary files

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2. Fig.. 1. Scheme of experiment. Subgroup 1 – control, subgroup 2 – PTSD model + saline injections, subgroup 3 – PTSD model + dalargin injections.

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3. Fig. 2. Adrenal cortex of rats from the ANT group, stained with iron hematoxylin using the Heidenhain method. I (a, d) – subgroup 1 (control); II (b, e) – subgroup 2 (PTSD model + saline injections); III (c, f) – subgroup 3 (PTSD model + dalargin injections); (a, b, c) – uv. 20; (d, e, f) – uv. 40.

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4. Fig.3. Morphometric parameters of the adrenal glands of rats of the ANT group

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5. Fig.4. Adrenal cortex of rats from the ABT group, stained with iron hematoxylin using the Heidenhain method. I (a, d) – subgroup 1 (control); II (b, e, g) – subgroup 2 (PTSD model + saline injections); III (c, f) – subgroup 3 (PTSD model + dalargin injections); (a, b, c) – uv. 20; (d, e, f) – uv. 40; (g) – uv. 100.

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6. Fig.5. Morphometric parameters of the adrenal glands of rats from the AVT group

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7. Fig.6. Adrenal cortex of rats from the PNT group, stained with iron hematoxylin using the Heidenhain method. I (a, d, g) – subgroup 1 (control); II (b, e) – subgroup 2 (PTSD model + saline injections); III (c, f) – subgroup 3 (PTSD model + dalargin injections); (a) – uv. 20; (b, c, d, g) – uv. 40; (e, f) – uv. 100.

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8. Fig.7. Morphometric parameters of the adrenal glands of rats of the PNT group

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9. Fig.8. Adrenal cortex of rats in the HTP group, stained with iron hematoxylin using the Heidenhain method. I (a, d) – subgroup 1 (control); II (b, e) – subgroup 2 (PTSD model + saline injections); III (c, f) – subgroup 3 (PTSD model + dalargin injections); (a, b) – uv. 20; (c, d, e) – uv. 40; (f) – uv. 100.

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10. Fig.9. Morphometric parameters of the adrenal glands of rats in the HTP group

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