Email this article Control Levels of Translocations in Human Peripheral Blood Lymphocyte Cultures Determined by Multicolor FISH Staining
- Authors: Nugis V.Y.1, Nikitina V.A.1, Lomonosova E.E.1, Kozlova M.G.1
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Affiliations:
- State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
- Issue: Vol 65, No 6 (2025)
- Pages: 614-624
- Section: РАДИАЦИОННАЯ ЦИТОГЕНЕТИКА
- URL: https://journals.rcsi.science/0869-8031/article/view/366281
- DOI: https://doi.org/10.7868/S3034590125060043
- ID: 366281
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Abstract
Introduction. In retrospective dose assessment using multicolor FISH staining, adequate dose assessment requires information on the average population levels of translocations in the control. Materials and Methods. Using the multi(24) color FISH method, chromosome aberrations were analyzed in peripheral blood lymphocyte cultures from 32 relatively healthy volunteer donors aged 21 to 72 years. Results. From 98 to 473 metaphases were analyzed in individual donors. The main quantity of FISH-registered chromosomal aberrations were reciprocal translocations with a frequency (per 100 cells) of 0.758 ± 0.118, which was significantly 5.9 times higher than the level of all unstable aberrations. The average frequencies of recorded translocations were 0.77 ± 0.13 and 0.74 ± 0.23 for women and men, respectively. Conclusion. In the control group, the observed frequencies of translocations in peripheral blood lymphocyte cultures did not have gender differences. Also, no significant effect of various types of medical diagnostic radiation, COVID-19 disease, and smoking on this cytogenetic indicator was found. A moderate correlation was observed between the frequencies of FISH translocations and the age of the donors.
About the authors
V. Yu. Nugis
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
Email: nugisvju@list.ru
ORCID iD: 0000-0001-7396-0246
Moscow
V. A. Nikitina
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
Email: nikitinava@yandex.ru
ORCID iD: 0000-0002-3150-7686
Moscow
E. E. Lomonosova
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
Email: mrs.lomonosova@gmail.com
ORCID iD: 0000-0003-3739-4261
Moscow
M. G. Kozlova
State Research Center — Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency
Email: koslovaamg@yandex.ru
ORCID iD: 0000-0001-8159-0513
Moscow
References
- Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies. Vienna: IAEA, 2011. 240 p.
- Ломоносова Е.Е., Нугис В.Ю., Козлова М.Г., Никитина В.А., Галстян И.А., Снигирёва Г.П. Трехцветный FISH-метод: сравнение ретроспективных цитогенетических дозовых оценок у разных пациентов, подвергшихся острому аварийному облучению. Радиационная биология. Радиоэкология. 2023; 63(3):240–249. [Lomonosova E.E., Nugis V. Yu., Kozlova M.G., Nikitina V.A., Galstyan I.A., Snigiryova G.P. Three-color FISH method: a comparison of retrospective cytogenetic dose estimations in different patients undergoing acute emergency irradiation. Radiation Biology. Radioecology. 2023; 63(3):240–249. (In Russ.)]. https://doi.org/10.31857/S0869803123030104
- Нугис В.Ю., Снигирёва Г.П., Ломоносова Е.Е., Козлова М.Г., Никитина В.А. Трехцветный FISH-метод: кривые доза-эффект для транслокаций в культурах лимфоцитов периферической крови после гамма-облучения in vitro. Медицинская радиология и радиационная безопасность. 2020; 65(5):12–20. [Nugis V. Yu., Snigiryova G.P., Lomonosova E.E., Kozlova M.G., Nikitina V.A. Three-color FISH-method: dose-effect curves for translocations in peripheral blood lymphocyte cultures after gamma-irradiation in vitro. Medical Radiology and Radiation Safety. 2020; 65(5):12–20. (In Russ.)]. https://doi.org/10.12737/1024-6177-2020-65-5-12-20
- Sigurdson A.J., Ha M., Hauptmann M., Bhattia P., Sram R.J., Beskid O., et al. International study of factors affecting human chromosome translocations. Mutat. Res. 2008; 652(2):112–121. https://doi.org/10.1016/j.mrgentox.2008.01.005
- Ломоносова Е.Е., Нугис В.Ю., Козлова М.Г., Никитина В.А. Контрольные уровни транслокаций в культурах лимфоцитов периферической крови людей, определенные с помощью трехцветного FISH-окрашивания. Радиационная биология. Радиоэкология. 2024; 64(6):585–597. [Lomonosova E.E., Nugis V.Yu., Kozlova M.G., Nikitina V.A. Control levels of tranlocations in human peripheral blood lymphocyte cultures determined using three-color FISH-staining. Radiation Biology. Radioecology. 2024; 64(6):585–597. (In Russ.)]. https://doi.org/10.31857/S0869803124060031
- Sotnik N.V., Osovets S.V., Scherthan H., Azizova T.V. mFISH analysis of chromosome aberrations in workers occupationally exposed to mixed radiation. Radiat. Environ. Biophys. 2014; 53(2):347–354. https://doi.org/10.1007/s00411-014-0536-7
- Нугис В.Ю., Козлова М.Г. Цитогенетические исследования в двух ситуациях обнаружения неконтролируемых источников ионизирующего излучения. Медицинская радиология и радиационная безопасность. 2015; 60(2):37–46.
- ISCN (2013): An International System for Human Cytogenetic Nomenclature, L.G. Shaffer, J. McGowan-jordan, M. Schmid (eds). Basel: S. Karger, 2013. 149 p.
- Ломоносова Е.Е., Нугис В.Ю., Никитина В.А., Козлова М.Г. Контрольные уровни FISH-регистрируемых транслокаций: обзор литературы. Радиационная биология. Радиоэкология. 2022; 62(4):399–407. [Lomonosova E.E., Nugis V. Yu., Nikitina V.A., Kozlova M.G. Control levels of Fish-registered translocations: literature review. Radiation Biology. Radioecology. 2022; 62(4):399–407. (In Russ.)]. https://doi.org/10.31857/S0869803122040075
- Гостенина В.И., Карандин К.С. Особенности применения корреляционного анализа в социологических исследованиях. Экономика. Социология. Право. 2017; 8(4):73–81. [Gostenina V.I., Karandin K.S. Peculiarities of the use of correlation analysis in sociological research. Economics. Sociology. Law. 2017; 8(4):73–81. (In Russ.)]. http://www.profit-brgu.ru
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