Isolation, Purification and some Properties of Staphylolytic Enzyme from Staphylococcus hyicus
- Authors: Fedorov T.V.1, Teymurazov M.G.1, Surin A.K.1, Tazina O.I.1, Biketov S.F.1
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Affiliations:
- “State Research Center for Applied Microbiology and Biotechnology” of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare
- Issue: Vol 59, No 5 (2023)
- Pages: 457-464
- Section: Articles
- URL: https://journals.rcsi.science/0555-1099/article/view/138812
- DOI: https://doi.org/10.31857/S0555109923050057
- EDN: https://elibrary.ru/WTWAXK
- ID: 138812
Cite item
Abstract
The paper presents data on the identification of a new staphylolytic enzyme from the cultural liquid of Staphylococcus hyicus B-8870. The primary sequence of the enzyme has the maximum similarity to the CHAP domain of N-acetylmuramoyl-L-alanine amidase from Staphylococcus sciuri DD 4747. The enzyme is active against a wide range of microorganisms of the Staphylococcus genus, including MRSA strains. The molecular weight of the enzyme is 13993 Da, the absorption coefficient at 280 nm is \(\varepsilon \frac{{{\text{mg}}}}{{{\text{ml}}}}\) 3.94, the value of the isoelectric point pI 10.35. The specific activity of the enzyme in relation to the cell suspension of S.aureus FDA 209P is 1518 U/mg with an optimum pH of 7.7 and a temperature of 40°C.
Keywords
About the authors
T. V. Fedorov
“State Research Center for Applied Microbiology and Biotechnology” of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare
Author for correspondence.
Email: tfedorov@yandex.ru
Russia, 142279, Serpukhov District, Moscow Region, Obolensk
M. G. Teymurazov
“State Research Center for Applied Microbiology and Biotechnology” of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare
Email: tfedorov@yandex.ru
Russia, 142279, Serpukhov District, Moscow Region, Obolensk
A. K. Surin
“State Research Center for Applied Microbiology and Biotechnology” of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare
Email: tfedorov@yandex.ru
Russia, 142279, Serpukhov District, Moscow Region, Obolensk
O. I. Tazina
“State Research Center for Applied Microbiology and Biotechnology” of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare
Email: tfedorov@yandex.ru
Russia, 142279, Serpukhov District, Moscow Region, Obolensk
S. F. Biketov
“State Research Center for Applied Microbiology and Biotechnology” of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare
Email: tfedorov@yandex.ru
Russia, 142279, Serpukhov District, Moscow Region, Obolensk
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