Preparation of Phage Antibodies to Heat Shock Proteins and Studying the Dynamics of their Accumulation in Mice with Xenotransplant Tumors

L. A. Dykman; Дыкман Л. А.; S. A. Staroverov; Староверов С. А.; R. D. Vyrshchikov; Вырщиков Р. Д.; K. K. Fursova; Фурсова К. К.; F. A. Brovko; Бровко Ф. А.; D. A. Soldatov; Солдатов Д. А.; O. I. Guliy; Гулий О. И.

doi:10.31857/S0555109923040050

Preparation of Phage Antibodies to Heat Shock Proteins and Studying the Dynamics of their Accumulation in Mice with Xenotransplant Tumors

Authors: Dykman L.A.¹, Staroverov S.A.¹^,2, Vyrshchikov R.D.¹, Fursova K.K.³, Brovko F.A.³, Soldatov D.A.², Guliy O.I.¹
Affiliations:
1. Institute of Biochemistry and Physiology of Plants and Microorganisms – Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences
2. Saratov State Vavilov Agrarian University
3. The Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Issue: Vol 59, No 4 (2023)
Pages: 410-417
Section: Articles
URL: https://journals.rcsi.science/0555-1099/article/view/138797
DOI: https://doi.org/10.31857/S0555109923040050
EDN: https://elibrary.ru/QYSAXC
ID: 138797

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Abstract
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Abstract

Using a naïve human scFv phage library, affinity selection of miniantibodies specific to heat shock proteins isolated from MH22a hepatoma cells and Sp2/0-Ag14 plasmacytoma cells was carried out. Using the obtained phage antibodies by dot-immunoassay and enzyme-linked immunosorbent assay, the dynamics of the concentration of heat shock proteins in the blood serum of mice with implanted tumor cells of the MH22a line was studied. Starting from the 14th day after xenotransplantation, there was a gradual increase in the level of heat shock proteins in the blood serum. It was found that after implantation of tumor cells, tumor growth was accompanied by a significant increase in the accumulation of heat shock proteins in the blood serum. It has been shown that miniantibodies specific to heat shock proteins are an effective tool for determining and monitoring the accumulation of heat shock proteins in the blood serum of animals.

Keywords

heat shock proteins, antibody phage library, tumor cell cultures, xenotransplanted tumors, immunoassay

References

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