Occurrence in sick animals and genetic heterogeneity of Siberian isolates of bovine respiratory syncytial virus (Pneumoviridae: Orthopneumovirus; BRSV) identified in the territories of the Ural, Siberian Federal District and the Republic of Kazakhstan
- 作者: Glotov A.1, Yuzhakov A.2, Glotova T.1, Nefedchenko A.1, Koteneva S.1, Komina A.2, Zhukova E.2
-
隶属关系:
- Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East
- Federal Scientific Center All-Russian Research Institute of Experimental Veterinary named after the honorary K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
- 期: 卷 69, 编号 1 (2024)
- 页面: 76-87
- 栏目: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/254061
- DOI: https://doi.org/10.36233/0507-4088-216
- EDN: https://elibrary.ru/eborho
- ID: 254061
如何引用文章
全文:
详细
Introduction. Bovine respiratory syncytial virus (Pneumoviridae: Orthornavirae, Orthopneumovirus; Bovine orthopneumovirus, Bovine respiratory syncytial virus, BRSV) is one of causative agents of respiratory diseases in animals. The study of the occurrence and genetic diversity of this pathogen is of particular importance.
Objective. To study the frequency of virus in animals using RT-PCR and genetic heterogeneity of isolates based on determining the complete nucleotide sequence of glycoprotein G gene.
Materials and methods. A 381-bp region of glycoprotein F gene was used for identification of virus genome, while complete nucleotide sequences of G gene were used for phylogenetic analysis. Phylogenetic trees were constructed using the maximum likelihood method in MEGA 7.0 software.
Results. During outbreaks of respiratory diseases, BRSV RNA was detected in animals of all ages in samples of lungs, nasal secretions, pulmonary lymph nodes. Complete nucleotide sequences of glycoprotein G gene, 771 bp in length were obtained for five isolates and 789 bp in length ‒ for two isolates. Nucleotide similarity between them was 87–100%. Phylogenetic analysis assigned the isolates to subgroups II and III, each of which included two isolates. A separate clade formed by K18 isolate from animals imported from Canada and sequences from vaccines containing the attenuated «375» strain.
Conclusion. The virus genome was identified in cows and heifers (20.0 and 14.3%), in calves up 1 month of age (3.05%), and in calves from 1 to 6 months of age (6.7%). Complete G gene nucleotide sequence analysis is a useful tool for studying the molecular epidemiology of BRSV on particular territories.
作者简介
Alexander Glotov
Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East
编辑信件的主要联系方式.
Email: glotov_vet@mail.ru
ORCID iD: 0000-0002-2006-0196
D. Sci. (Vet.), Professor, Chief Researcher – Head of Biotechnology Laboratory, Diagnostic Center, Siberian Federal Scientific Center of Agrobiotechnologies (SFSCA) of the Russian Academy of Sciences, Institute of Experimental Veterinary Medicine of Siberia and the Far
俄罗斯联邦, 630501, Krasnoobsk, Novosibirsk regionAnton Yuzhakov
Federal Scientific Center All-Russian Research Institute of Experimental Veterinary named after the honorary K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
Email: anton_oskol@mail.ru
ORCID iD: 0000-0002-0426-9678
Ph.D. biol. Sciences, head of laboratory, Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
俄罗斯联邦, 109428, MoscowTatyana Glotova
Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East
Email: t-glotova@mail.ru
ORCID iD: 0000-0003-3538-8749
SPIN 代码: 7488-5915
Sci. (Biol.), Professor, Chief Researcher – Biotechnology Laboratory-Diagnostic Center Siberian Federal Scientific Center of Agrobiotechnologies (SFSCA) of the Russian Academy of Sciences, Institute of Experimental Veterinary Medicine of Siberia and the Far East
俄罗斯联邦, 630501, Krasnoobsk, Novosibirsk regionAlexey Nefedchenko
Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East
Email: homeovet@narod.ru
ORCID iD: 0000-0002-4181-4268
SPIN 代码: 1583-5776
D. Sci. (Vet.), Associate Professor, Leading Researcher of the laboratory of biotechnology – diagnostic center of the Siberian Federal Scientific Center of Agrobiotechnologies (SFSCA) of the Russian Academy of Sciences, Institute of Experimental Veterinary Medicine of Siberia and the Far East
俄罗斯联邦, 630501, Krasnoobsk, Novosibirsk regionSvetlana Koteneva
Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East
Email: koteneva-sv@mail.ru
ORCID iD: 0000-0003-2649-7505
SPIN 代码: 7545-7206
Ph.D. Sci (Vet.), leading researcher at the laboratory of biotechnology-diagnostic center of the Siberian Federal Scientific Center for Agrobiotechnologies (SFSCA) of the Russian Academy of Sciences, Institute of Experimental Veterinary Medicine of Siberia and the Far East
俄罗斯联邦, 630501, Krasnoobsk, Novosibirsk regionAlina Komina
Federal Scientific Center All-Russian Research Institute of Experimental Veterinary named after the honorary K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
Email: komina.a.k@yandex.ru
ORCID iD: 0000-0002-7173-5501
SPIN 代码: 3699-2612
graduate student, Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
俄罗斯联邦, 109428, MoscowElena Zhukova
Federal Scientific Center All-Russian Research Institute of Experimental Veterinary named after the honorary K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
Email: evz-sk@mail.ru
ORCID iD: 0000-0001-7423-6102
Ph.D. biol. Sciences, leading researcher at the Laboratory of Biochemistry and Molecular Biology Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Y.R. Kovalenko of the Russian Academy of Sciences
俄罗斯联邦, 109428, Moscow参考
- Cummings D.B., Meyer N.F., Step D.L. Bovine respiratory disease considerations in young dairy calves. Vet. Clin. North Am. Food Anim. Pract. 2022; 38(1): 93–105. https://doi.org/10.1016/j.cvfa.2021.11.007
- Gorden P.J., Plummer P. Control, management, and prevention of bovine respiratory disease in dairy calves and cows. Vet. Clin. North Am. Food Anim. Pract. 2010; 26(2): 243–59. https://doi.org/10.1016/j.cvfa.2010.03.004
- Valarcher J.F., Taylor G. Bovine respiratory syncytial virus infection. Vet. Res. 2007; 38(2): 153–80. https://doi.org/10.1051/vetres:2006053
- Kirolos A., Christides A., Xian S., Reeves R., Nair H., Campbell H. A landscape review of the published research output relating to respiratory syncytial virus (RSV) in North & Central America and Europe between 2011–2015. J. Glob. Health. 2019; 9(1): 010425. https://doi.org/10.7189/jogh.09.010425
- Renault V., Damiaans B., Sarrazin S., Humblet M.F., Lomba M., Ribbens S., et al. Classification of adult cattle infectious diseases: A first step towards prioritization of biosecurity measures. Transbound. Emerg. Dis. 2018; 65(6): 1991–2005. https://doi.org/10.1111/tbed.12982
- Makoschey B., Berge A.C. Review on bovine respiratory syncytial virus and bovine parainfluenza – usual suspects in bovine respiratory disease – a narrative review. BMC Vet. Res. 2021; 17(1): 261. https://doi.org/10.1186/s12917-021-02935-5
- Amarasinghe G.K., Bào Y., Basler C.F., Bavari S., Beer M., Bejerman N., et al. Taxonomy of the order Mononegavirales: update 2017. Arch. Virol. 2017; 162(8): 2493–504. https://doi.org/10.1007/s00705-017-3311-7
- Rima B., Collins P., Easton A., Fouchier R., Kurath G., Lamb R.A., et al. ICTV Report Consortium. ICTV virus taxonomy profile: Pneumoviridae. J. Gen. Virol. 2017; 98(12): 2912–3. https://doi.org/10.1099/jgv.0.000959.
- Larsen L.E. Bovine respiratory syncytial virus (BRSV): a review. Acta Vet. Scand. 2000; 41(1): 1–24. https://doi.org/10.1186/bf03549652
- Fulton R.W., Purdy C.W., Confer A.W., Saliki J.T., Loan R.W., Briggs R.E., et al. Bovine viral diarrhea viral infections in feeder calves with respiratory disease: interactions with Pasteurella spp., parainfluenza-3 virus, and bovine respiratory syncytial virus. Can. J. Vet. Res. 2000; 64(3): 151–9.
- Guzman E., Taylor G. Immunology of bovine respiratory syncytial virus in calves. Mol. Immunol. 2015; 66(1): 48–56. https://doi.org/10.1016/j.molimm.2014.12.004
- Larsen L.E., Tjørnehøj K., Viuff B. Extensive sequence divergence among bovine respiratory syncytial viruses isolated during recurrent outbreaks in closed herds. J. Clin. Microbiol. 2000; 38(11): 4222–7. https://doi.org/10.1128/jcm.38.11.4222-4227.2000
- Sarmiento-Silva R.E., Nakamura-Lopez Y., Vaughan G. Epidemiology, molecular epidemiology and evolution of bovine respiratory syncytial virus. Viruses. 2012; 4(12): 3452–67. https://doi.org/10.3390/v4123452
- Leme R.A., Dall Agnol A.M., Balbo L.C., Pereira F.L., Possatti F., Alfieri A.F., et al. Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus. Vet Q. 2020; 40(1): 83–96. https://doi.org/10.1080/01652176.2020.1733704
- Sacco R.E., McGill J.L., Pillatzki A.E., Palmer M.V., Ackermann M.R. Respiratory syncytial virus infection in cattle. Vet. Pathol. 2014; 51(2): 427–36. https://doi.org/10.1177/0300985813501341
- Valentova V. The antigenic and genetic variability of bovine respiratory syncytial virus with emphasis on the G protein. Veterinární medicína. 2012; 48(9): 254–66. https://doi.org/10.17221/5778-VETMED18
- Doreleijers J.F., Langedijk J.P.M., Hård K., Boelens R., Rull- mann J.A.C., Schaaper W.M., et al. Solution structure of the immunodominant region of protein G of bovine respiratory syncytial virus. Biochemistry. 1996; 35(47): 14684–8. https://doi.org/10.1021/bi9621627
- Valarcher J.F., Schelcher F., Bourhy H. Evolution of bovine respiratory syncytial virus. J. Virol. 2000; 74(22): 10714–28. https://doi.org/10.1128/jvi.74.22.10714-10728.2000
- Furze J.M., Roberts S.R., Wertz G.W., Taylor G. Antigenically distinct G glycoproteins of BRSV strains share a high degree of genetic homogeneity. Virology. 1997; 231(1): 48–58. https://doi.org/10.1006/viro.1997.8490
- Krešić N., Bedeković T., Brnić D., Šimić I., Lojkić I., Turk N. Genetic analysis of bovine respiratory syncytial virus in Croatia. Comp. Immunol. Microbiol. Infect. Dis. 2018; 58: 52–7. https://doi.org/10.1016/j.cimid.2018.09.004
- Giammarioli M., Mangili P., Nanni A., Pierini I., Petrini S., Pirani S., et al. Highly pathogenic Bovine Respiratory Syncytial virus variant in a dairy herd in Italy. Vet. Med. Sci. 2020; 6(4): 740–5. https://doi.org/10.1002/vms3.312
- Klem T.B., Rimstad E., Stokstad M. Occurrence and phylogenetic analysis of bovine respiratory syncytial virus in outbreaks of respiratory disease in Norway. BMC Vet. Res. 2014; 10(1): 15. https://doi.org/10.1186/1746-6148-10-15
- Bertolotti L., Giammarioli M., Rosati S. Genetic characterization of bovine respiratory syncytial virus strains isolated in Italy: evidence for the circulation of new divergent clades. J. Vet. Diagn. Invest. 2018; 30(2): 300–4. https://doi.org/10.1177/1040638717746202
- Bidokti M.R., Tråvén M., Ohlson A., Zarnegar B., Baule C., Belák S., et al. Phylogenetic analysis of bovine respiratory syncytial viruses from recent oubreaks in feedlot and dairy cattle herds. Arch. Virol. 2012; 157(4): 601–7. https://doi.org/10.1007/s00705-011-1209-3
- Jia S., Yao X., Yang Y., Niu C., Zhao Y., Zhang X., et al. Isolation, identification, and phylogenetic analysis of subgroup III strain of bovine respiratory syncytial virus contributed to outbreak of acute respiratory disease among cattle in Northeast China. Virulence. 2021; 12(1): 404–14. https://doi.org/10.1080/21505594.2021.1872178
- Chang Y., Yue H., Tang C. Prevalence and molecular characteristics of bovine respiratory syncytial virus in beef cattle in China. Animals (Basel). 2022; 12(24): 3511. https://doi.org/10.3390/ani12243511
- Karayel Hacioğlu İ., Coşkun N., Duran Yelken S., Sevinç S., Alkan F. Phylogenetic analysis of bovine respiratory syncytial viruses from calves with respiratory disorders. Kafkas Univ. Vet. Fak. Derg. 2019; 25(2): 251–6. https://doi.org/10.9775/kvfd.2018.20819
- Ellis J., Marx J., Perumbakkam S., West K., Gow S., Lacoste S., et al. Genealogy of an in-vivo passaged isolate of western Canadian bovine respiratory syncytial virus. Can. J. Vet. Res. 2022; 86(3): 218–28.
- Nettleton P.F., Gilray J.A., Caldow G., Gidlow J.R., Durkovic B., Vilcek S. Recent isolates of bovine respiratory syncytial virus from Britain are more closely related to isolates from the USA than to earlier British and current mainland European isolates. J. Vet. Med. B Infect. Dis. Vet. Public Health. 2003; 50(4): 196–9. https://doi.org/10.1046/j.1439-0450.2003.00647.x
- Krešić N., Bedeković T., Brnić D., Šimić I., Lojkić I., Turk N. Genetic analysis of bovine respiratory syncytial virus in Croatia. Comp. Immunol. Microbiol. Infect. Dis. 2018; 58: 52–7. https://doi.org/10.1016/j.cimid.2018.09.004
- Almeida R.S., Domingues H.G., Spilki F.R., Larsen L.E., Hägglund S., Belák S., et al. Circulation of bovine respiratory syncytial virus in Brazil. Vet. Rec. 2006; 158(18): 632–4. https://doi.org/10.1136/vr.158.18.632
- Kumagai A., Kawauchi K., Andoh K., Hatama S. Sequence and unique phylogeny of G genes of bovine respiratory syncytial viruses circulating in Japan. J. Vet. Diagn. Investig. 2021; 33(1): 162–6. https://doi.org/10.1177/1040638720975364
- Glotov A.G., Glotova T.I., Koteneva S.V., Nefedchenko A.V. Synthetic oligonucleotide primers and method of bovine respiratory syncytial infection RNA virus detection by synthetic oligonucleotide primers in Polymerase Chain Reaction (PCR). Patent RF 2405039 C1; 2010. https://elibrary.ru/ttzyly (in Russian)
- Prozzi D., Walravens K., Langedijk J.P., Daus F., Kramps J.A., Letesson J.J. Antigenic and molecular analyses of the variability of bovine respiratory syncytial virus G glycoprotein. J. Gen. Virol. 1997; 78(Pt. 2): 359–66. https://doi.org/10.1099/0022-1317-78-2-359
- Langedijk J.P., Meloen R.H., Taylor G., Furze J.M., van Oirschot J.T. Antigenic structure of the central conserved region of protein G of bovine respiratory syncytial virus. J. Virol. 1997; 71(5): 4055–61. https://doi.org/10.1128/jvi.71.5.4055-4061.1997
- Glotov A.G., Glotova T.I., Koteneva S.V., Nefedchenko A.V., Voytova K.V. Features of epidemiological situation on bovine respiratory syncytial virus infection (BRSV) in dairy farms. Veterinariya. 2010; (7): 21–5. https://elibrary.ru/msrezd (in Russian)
- Nefedchenko A.V., Glotov A.G., Koteneva S.V., Glotova T.I. Developing and testing a real-time polymerase chain reaction to identify and quantify bovine respiratory syncytial viruses. Molekulyarnaya genetika, mikrobiologiya i virusologiya. 2020; 38(3): 145–50. https://doi.org/10.17116/molgen202038031145 https://elibrary.ru/obaant (in Russian)
- Nefedchenko A.V., Glotov A.G., Koteneva S.V., Glotova T.I. Detection and quantitative assessment of viral and bacterial pathogens in bovine respiratory diseases by real-time-qPCR. Sel’skokhozyaystvennaya biologiya. 2021; 56(4): 695–706. https://doi.org/10.15389/agrobiology.2021.4.695rus https://elibrary.ru/spttqp (in Russian)