Molecular and biological properties of the African swine fever virus (Asfarviridae: Asfivirus) isolate ASF/Tatarstan 20/WB-12276

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Abstract

Introduction. Up-to-date data and full characterization of circulating ASFV isolates play a crucial role in virus eradication and control in endemic regions and countries.

The aim of the study was to evaluate and characterize the molecular and biological properties of the ASFV isolate ASF/Tatarstan 20/WB-12276, conduct phylogenetic analysis, and compare the results with isolates circulating in Europe and Asia.

Materials and methods. For bioassay, eight heads of the Large White pigs weighing 15–20 kg/head were used. Detection of specific anti-ASFV antibodies by ELISA and immunoperoxidase method. Detection of ASFV genome was performed by qPCR. Isolation of ASF/Tatarstan 20/WB-12276 and determination of titer were performed in pig spleen cell culture. Sequencing was carried out by the Sanger method.

Results. The virus was characterized as highly virulent and capable of causing acute to subacute forms of ASF. Phylogenetic analysis revealed substitutions in the genome of the ASF/Tatarstan 20/WB-12276 isolate (IGR/I73R-I329L and I267L markers) that supported the clustering of the studied variant with isolates prevalent in most of Europe and Asia.

Conclusion. For the first time, the molecular and biological properties of the ASF/Tatarstan 20/WB-12276 virus isolate taken from a wild boar shot on the territory of the Republic of Tatarstan were studied and analyzed.

About the authors

Andrey R. Shotin

Federal Center for Animal Health (ARRIAH)

Author for correspondence.
Email: shotin@arriah.ru
ORCID iD: 0000-0001-9884-1841

Candidate of Science (Veterinary Medicine), Researcher, Reference Laboratory for African Swine Fever

Russian Federation, 600901, Vladimir

Roman S. Chernyshev

Federal Center for Animal Health (ARRIAH)

Email: chernishev_rs@arriah.ru
ORCID iD: 0000-0003-3604-7161

Postgraduate Student, Veterinarian, Reference Laboratory for African Swine Fever

Russian Federation, 600901, Vladimir

Elizaveta O. Morozova

Federal Center for Animal Health (ARRIAH)

Email: morozova_eo@arriah.ru
ORCID iD: 0000-0002-0955-9586

Postgraduate Student, Biologist, Reference Laboratory for African Swine Fever

Russian Federation, 600901, Vladimir

Alexey S. Igolkin

Federal Center for Animal Health (ARRIAH)

Email: igolkin_as@arriah.ru
ORCID iD: 0000-0002-5438-8026

Candidate of Science (Veterinary Medicine), Head of Reference Laboratory for African swine fever

Russian Federation, 600901, Vladimir

Konstantin N. Gruzdev

Federal Center for Animal Health (ARRIAH)

Email: gruzdev@arriah.ru
ORCID iD: 0000-0003-3159-1969

Doctor of Science (Biology), Professor, Chief Researcher, Information and Analysis Centre

Russian Federation, 600901, Vladimir

Ivan S. Kolbin

Federal Center for Animal Health (ARRIAH)

Email: kolbin@arriah.ru
ORCID iD: 0000-0003-4692-1297

Postgraduate Student, Veterinarian, Reference Laboratory for African Swine Fever

Russian Federation, 600901, Vladimir

Ivan A. Lavrentiev

Federal Center for Animal Health (ARRIAH)

Email: lavrentev@arriah.ru
ORCID iD: 0009-0003-0552-3812

Postgraduate Student, Leading Veterinarian, Reference Laboratory for African Swine Fever

Russian Federation, 600901, Vladimir

Ali Mazloum

Federal Center for Animal Health (ARRIAH)

Email: mazlum@arriah.ru
ORCID iD: 0000-0002-5982-8393

Candidate of Science (Biology), Senior Researcher, Reference Laboratory for African Swine Fever

Russian Federation, 600901, Vladimir

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3. Fig. 1. Results of body temperature measurements and testing of blood samples from infected pigs (№№ 1–3) by real-time PCR (qPCR) and ELISA (n = 3). Note: † – death date; the horizontal line across the temperature axis marks the limit of the physiological normal body temperature (40.0 °С); the green bars correspond for the qPCR Ct values.

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4. Fig. 2. Results of body temperature measurements and testing of blood samples from infected pigs (№№ 4–6) by qPCR, ELISA, and IPM (n = 3). † – death date; the horizontal line across the temperature axis marks the limit of the physiological normal body temperature (40.0 °С); the bars correspond for the qPCR Ct values; the vertical dotted line across the dates shows the first positive IPM result.

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5. Fig. 3. Results of body temperature measurements and testing of blood samples from infected pigs (№№ 7, 8) by qPCR, ELISA, and IPM (n = 3) † – death date; the horizontal line across the temperature axis marks the limit of the physiological normal body temperature (40.0 °С); the bars correspond for the qPCR Ct values; the vertical dotted line across the dates shows the first positive IPM result.

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Copyright (c) 2023 Shotin A.R., Chernyshev R.S., Morozova E.O., Igolkin A.S., Gruzdev K.N., Kolbin I.S., Lavrentiev I.A., Mazloum A.

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