Antigenic and immunogenic activity of virus-like particles based on rabbit hemorrhagic disease virus (Caliciviridae: Lagovirus) genotypes GI1 and GI2 recombinant major capsid proteins
- 作者: Mukhin A.N.1, Alekseev K.P.1, Yuzhakov A.G.2, Selezneva E.V.1, Moskvina A.S.3, Verkhovsky O.A.3, Aliper T.I.1
-
隶属关系:
- Vetbiochem LLC
- Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences
- Research Institute for Diagnosis and Prevention of Human and Animal Diseases
- 期: 卷 68, 编号 2 (2023)
- 页面: 132-141
- 栏目: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/132625
- DOI: https://doi.org/10.36233/0507-4088-164
- EDN: https://elibrary.ru/rswvjz
- ID: 132625
如何引用文章
全文:
详细
Introduction. Rabbit hemorrhagic disease is an acute highly contagious infection associated with two genotypes of pathogenic Lagovirus. Antibodies to major capsid protein (Vp60) are protective.
The aim of the work ‒ is an evaluation of antigenic and immunogenic activity of virus-like particles (VLPs) based on recombinant major capsid proteins of both genotypes of rabbit hemorrhagic disease virus (RHDV) (recVP60-GI1 and recVP60-GI2).
Materials and methods. Baculovirus-expressed VLPs were evaluated using electron microscopy and administered to clinically healthy 1.5–3 month old rabbits in a dose of 50 µg. Rabbits were challenged with 103 LD50 of virulent strains “Voronezhsky-87” and “Tula” 21 days post immunization. Serum samples were tested for the presence of RHDV-specific antibodies.
Results. VLPs with hemagglutination activity forming VLP 30–40 nm in size were obtained in Hi-5 cell culture. Specific antibody titers in rabbits measured by ELISA were 1 : 200 to 1 : 800 on 21th day post immunization with VLPs. Immunogenic activity of recVP60-GI1 VLPs was 90 and 40%, while it was 30 and 100% for recVP60-GI2 VLPs after the challenge with RHDV genotypes 1 and 2 respectively. The immunogenicity of two VLPs in mixture reached 100%.
Discussion. VLPs possess hemagglutinating, antigenic and immunogenic activity, suggesting their use as components in substances designed for RHDV specific prophylaxis in rabbits. Results of the control challenge experiment demonstrated the need to include the antigens from both RHDV genotypes in the vaccine.
Conclusion. Recombinant proteins recVP60-GI1 and recVP60-GI2 form VLPs that possess hemagglutinating an antigenic activity, and provide 90–100% level of protection for animals challenged with RHDV GI1 and GI2 virulent strains.
作者简介
Aleksey Mukhin
Vetbiochem LLC
编辑信件的主要联系方式.
Email: amuhin@yahoo.com
ORCID iD: 0000-0001-7008-5212
Ph.D. (Biol.), Leading Researcher
俄罗斯联邦, 109316, MoscowKonstantin Alekseev
Vetbiochem LLC
Email: kalekseev@hotmail.com
ORCID iD: 0000-0001-9536-3127
Ph.D. (Biol.), Leading Researcher
俄罗斯联邦, 109316, MoscowAnton Yuzhakov
Federal Scientific Center – All-Russian Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences
Email: anton_oskol@mail.ru
ORCID iD: 0000-0002-0426-9678
Ph.D. (Biol.), Head of laboratory
俄罗斯联邦, 109428, MoscowEkaterina Selezneva
Vetbiochem LLC
Email: selja07@rambler.ru
ORCID iD: 0000-0002-5592-2948
microbiologist of department of quality control
俄罗斯联邦, 109316, MoscowAnna Moskvina
Research Institute for Diagnosis and Prevention of Human and Animal Diseases
Email: annamoskvina17@gmail.com
ORCID iD: 0000-0002-4542-8196
Ph.D. (Biol.), Leading Researcher
俄罗斯联邦, 123098, MoscowOleg Verkhovsky
Research Institute for Diagnosis and Prevention of Human and Animal Diseases
Email: info@dpri.com
ORCID iD: 0000-0003-0784-9341
Professor, President
俄罗斯联邦, 123098, MoscowTaras Aliper
Vetbiochem LLC
Email: coronavirusr@yandex.ru
ORCID iD: 0000-0003-2696-1363
Professor, Director of science and innovation
俄罗斯联邦, 109316, Moscow参考
- Liu S.J., Xue H.P., Pu B.Q., Quian N.H. A new viral disease in rabbits. Anim. Husb. Vet. Med. 1984; 16(6): 253–5.
- Mitro S., Krauss H. Rabbit hemorrhagic disease: a review with special reference to its epizootiology. Eur. J. Epidemiol. 1993; 9(1): 70–8. https://doi.org/10.1007/bf00463093
- Puggioni G., Cavadini P., Maestrale C., Scivoli R., Botti G., Ligios C., et al. The new French 2010 Rabbit Hemorrhagic Disease Virus causes an RHD-like disease in the Sardinian Cape hare (Lepus capensis mediterraneus). Vet. Res. 2013; 44(1): 96. https://doi.org/10.1186/1297-9716-44-96
- Abrantes J., Lopes A.M., Dalton K.P., Melo P., Correia J.J., Ramada M., et al. New variant of rabbit hemorrhagic disease virus, Portugal, 2012-2013. Emerg. Infect. Dis. 2013; 19(11): 1900–1902. https://doi.org/10.3201/eid1911.130908
- Dalton K.P., Nicieza I., Abrantes J., Esteves P.J., Parra F. Spread of new variant RHDV in domestic rabbits on the Iberian Peninsula. Vet. Microbiol. 2014; 169(1-2): 67–73. https://doi.org/10.1016/j.vetmic.2013.12.015
- Duarte M., Henriques M., Barros S.C., Fagulha T., Ramos F., Luis T., et al. Detection of RHDV variant 2 in domestic rabbits in Azores. Vet. Rec. 2015; 176(19): 499–500. https://doi.org/10.1136/vr.h2402
- OIE. Rabbit Haemorrhagic Disease. USA; 2020. Available at: https://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_refer=MapEventSummary&reportid=34728
- Le Pendu J., Abrantes J., Bertagnoli S., Guitton J.S., Le Gall-Reculé G., Lopes A.M., et al. Proposal for a unified classification system and nomenclature of lagoviruses. J. Gen. Virol. 2017; 98(7): 1658–66. https://doi.org/10.1099/jgv.0.000840
- Mukhin A.N., Yuzhakov A.G., Selezneva E.V., Drozdova E.I., Verkhovskiy O.A., Aliper T.I. Outbreak of the disease caused by the rabbit hemorrhagic disease virus 2 in the Russian Federation. Agrarnaya nauka. 2021; (4): 25–7. https://doi.org/10.32634/0869-8155-2021-348-4-25-27 EDN: https://www.elibrary.ru/ynmqnh (in Russian)
- Burmakina G., Malogolovkina N., Lunitsin A., Titov I., Tsybanov S., Malogolovkin A. Comparative analysis of rabbit hemorrhagic disease virus strains originating from outbreaks in the Russian Federation. Arch. Virol. 2016; 161(7): 1973–9. https://doi.org/10.1007/s00705-016-2864-1
- Federal Research Center for Virology and Microbiology. The third case of detection of a new type of rabbit hemorrhagic disease virus – VGBK-2 in the Russian Federation. Available at: https://ficvim.ru/2019/02/tretij-sluchaj-obnaruzheniya-virusa-gemorragicheskoj-bolezni-krolikov-novogo-tipa-vgbk-2-v-rossijskoj-federacii/ (in Russian)
- Le Gall-Recule G., Lavazza A., Marchandeau S., Bertagnoli S., Zwingelstein F., Cavadini P., et al. Emergence of a new lagovirus related to Rabbit haemorrhagic disease virus. Vet. Res. 2013; 44(1): 81. https://doi.org/10.1186/1297-9716-44-81
- Peacock D., Kovaliski J., Sinclair R., Mutze G., Iannella A., Capucci L. RHDV2 overcoming RHDV immunity in wild rabbits (Oryctolagus cuniculus) in Australia. Vet. Rec. 2017; 180(11): 280. https://doi.org/10.1136/vr.104135
- Vlasova N.N., Vlasov N.A., Alekseev K.P. Construction of plasmid vector of expressing virus of haemorrhagic disease of rabbits. Veterinariya. 2006; (11): 53–5. https://www.elibrary.ru/hvjxut (in Russian)
- Gromadzka B., Szewczyk B., Konopa G., Fitzner A., Kesy A. Recombinant VP60 in the form of virion-like particles as a potential vaccine against rabbit hemorrhagic disease virus. Acta Biochim. Pol. 2006; 53(2): 371–6.
- Guo H., Zhu J., Tan Y., Li C., Chen Z., Sun S., et al. Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits. Antiviral Res. 2016; 131: 85–91. https://doi.org/10.1016/j.antiviral.2016.04.011
- López-Vidal J., Gómez-Sebastián S., Bárcena J., Nuñez Mdel C., Martínez-Alonso D., Dudognon B., et al. Improved production efficiency of virus-like particles by the baculovirus expression vector system. PLoS One. 2015; 10(10): e0140039. https://doi.org/10.1371/journal.pone.0140039
- Qi R., Miao Q., Zhu J., Tang J., Tang A., Wang X., et al. Construction and immunogenicity of novel bivalent virus-like particles bearing VP60 genes of classic RHDV(GI.1) and RHDV2(GI.2). Vet. Microbiol. 2020; 240: 108529. https://doi.org/10.1016/j.vetmic.2019.108529
- Alekseev K.P., Moskvina A.S., Verkhovskiy O.A., Selezneva E.V., Chernykh O.Yu., Mukhin A.N. Obtaining recombinant capsid protein VP60 of rabbit haemorrhagic disease virus and its antigenic and immunogenic activity study. Veterinariya Kubani. 2020; (5): 34–7. https://doi.org/10.33861/2071-8020-2020-5-34-37 EDN: https://www.elibrary.ru/vxzgjm (in Russian)
- Selezneva E.V., Mukhin A.N., Ezdakova I.Yu., Verkhovsky O.A., Aliper T.I. The application of recombinant Vp60-based ELISA for haemorrhagic disease virus antibody detection to vaccination against RHD. AIP Conf. Proc. 2022; 2467: 070028-1–070028-5. https://doi.org/10.1063/5.0092529
- Pawlikowska M., Hukowska-Szematowicz B., Deptuła W. Phylogenetic analysis of selected strains of Rabbit haemorrhagic disease virus on the basis of N-terminal fragment of the gene encoding structural protein VP60. Bull. Vet. Inst. Pulawy. 2010; 54: 129–33.
- Dalton K.P., Arnal J.L., Benito A.A., Chacón G., Martín Alonso J.M., Parra F. Conventional and real time RT-PCR assays for the detection and differentiation of variant rabbit hemorrhagic disease virus (RHDVb) and its recombinants. J. Virol. Methods. 2018; 251: 118–22. https://doi.org/10.1016/j.jviromet.2017.10.009
补充文件
