Mumps virus (Paramyxoviridae: Orthorubulavirus: Mumps orthorubulavirus) genotyping as a component of laboratory confirmation of infection

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Abstract

Introduction. Mumps is a viral infection of high social significance. National program «Elimination of measles and rubella and achievement of a stable sporadic incidence of epidemic mumps in the Russian Federation (2021–2025)» sets the aim of gradual integration of mumps surveillance into the existing measles and rubella surveillance system. One of the key components of surveillance system is a laboratory confirmation of mumps cases. There are two approaches for laboratory confirmation of mumps cases, based on serological or molecular genetic methods. The aim of the work is molecular genetic characteristic of the mumps viruses (MuVs) circulated in the Russian Federation in 2022.

Materials and methods. Samples of swabs from the inner surface of the cheek of 11 patients with mumps were collected for the study. Viral RNA was isolated directly from the samples. The isolated RNA was used as a matrix for RT-PCR. PCR products were sequenced using the Sanger method, and phylogenetic analysis was performed using the MEGA-X software.

Results. The MuV genotype G was detected in all samples. Phylogenetic analysis showed the presence of two virus genetic groups G-1 and G-2 that were significantly different from the viruses circulating in other countries.

Conclusion. The identification of two MuV genetic groups in a limited area suggests a high genetic diversity of the pathogen.

About the authors

T. S. Rubalskaia

Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Author for correspondence.
Email: rubalskaia@gabrich.ru
ORCID iD: 0000-0003-0838-7353

Head of Applied Immunochemistry Laboratory

Russian Federation, 125212, Moscow

D. V. Erokhov

Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: erokhovdenis@gmail.com
ORCID iD: 0000-0001-7163-7840

Research Associate of Applied Immunochemistry Laboratory

Russian Federation, 125212, Moscow

P. E. Zherdeva

Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: polya-zherdeva@mail.ru
ORCID iD: 0000-0002-7635-4353

Junior Research Associate of Applied Immunochemistry Laboratory

Russian Federation, 125212, Moscow

A. V. Milikhina

Center for Hygiene and Epidemiology in the Republic of Dagestan

Email: milihina_alina@mail.ru
ORCID iD: 0000-0002-4831-2922

Head of the Virological Laboratory

Russian Federation, 367009, Makhachkala

A. A. Gadzhiewa

Center for Hygiene and Epidemiology in the Republic of Dagestan

Email: ai.gazhiewa@yandex.ru
ORCID iD: 0000-0002-1919-6483

Head of the Epidemiology Department

Russian Federation, 367009, Makhachkala

N. T. Tikhonova

Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: tikhmail@mail.ru
ORCID iD: 0000-0002-8762-4355

Professor, Dr. Sci. (Biology) Chief Research Associate of Cytokines Laboratory

Russian Federation, 125212, Moscow

References

  1. National Program «Elimination of measles and rubella and achievement of a stable sporadic incidence of epidemic mumps in the Russian Federation (2021–2025). Moscow; 2020. (in Russian)
  2. SanPiN 3.3686–21. Sanitary and epidemiological requirements for the prevention of infectious diseases. (in Russian)
  3. WHO. Mumps: Vaccine Preventable Diseases Surveillance Standards. Available at: https://www.who.int/publications/m/item/vaccine-preventable-diseases-surveillance-standards-mumps
  4. Chekhlyaeva T.S., Erokhov D.V., Andrievskaya I.Yu., Zherdeva P.E., Tikhonova N.T. Genetic diversity of the mumps viruses (Paramyxoviridae: Orthorubulavirus: Mumps orthorubulavirus): an overview. Voprosy virusologii. 2022; 67(2): 95–106. https://doi.org/10.36233/0507-4088-98 (in Russian)
  5. State report of the Chief Sanitary Doctor «On the state of sanitary and epidemiological welfare of the population in the Russian Federation in 2021»; 2021. Available at: https://www.rospotrebnadzor.ru/documents/details.php?ELEMENT_ID=21796 (in Russian)
  6. Newsletter «Incidence of measles, rubella, mumps in the Russian Federation for 2022 (6 months)». Available at: http://www.gabrich.ru/measles-center.html (accessed 14.12.2022) (in Russian)
  7. Lee J.Y., Kim Y.Y., Shin G.C., Na B.K., Lee J.S., Lee H.D., et al. Molecular characterization of two genotypes of mumps virus circulated in Korea during 1998–2001. Virus Res. 2003; 97(2): 111–6. https://doi.org/10.1016/j.virusres.2003.08.006
  8. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 1987; 4(4): 406–25. https://doi.org/10.1093/oxfordjournals.molbev.a040454
  9. Tamura K. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G+ C-content biases. Mol. Biol. Evol. 1992; 9(4): 678–87. https://doi.org/10.1093/oxfordjournals.molbev.a040752
  10. WHO. Mumps virus nomenclature update: 2012. Wkly Epidemiol. Rec. 2012; 87(22): 217–24.
  11. Li J., Claes Ö., Myers R., Rota P.A., Nakayama T., Forcic D., et al. Genomic diversity of mumps virus and global distribution of the 12 genotypes. Rev. Med. Virol. 2014; 25(2): 85–101. https://doi.org/10.1002/rmv.1819
  12. Afzal M.A., Buchanan J., Heath A.B., Minor P.D. Clustering of mumps virus isolates by SH gene sequence only partially reflects geographical origin. Arch. Virol. 1997; 142(2): 227–38. https://doi.org/10.1007/s007050050073
  13. Cohen C., White J.M., Savage E.J., Glynn J.R., Choi Y., Andrews N., et al. Vaccine effectiveness estimates, 2004–2005 mumps outbreak, England. Emerg. Infect. Dis. 2007; 13(1): 12–7. https://doi.org/10.3201/eid1301.060649
  14. Šantak M., Košutić-Gulija T., Tešović G., Ljubin-Sternak S., Gjenero-Margan I., Betica-Radić L., et al. Mumps virus strains isolated in Croatia in 1998 and 2005: Genotyping and putative antigenic relatedness to vaccine strains. J. Med. Virol. 2006; 78(5): 638–43. https://doi.org/10.1002/jmv.20587
  15. Carr M.J., Moss E., Waters A., Dean J., Jin L., Coughlan S., et al. Molecular epidemiological evaluation of the recent resurgence in mumps virus infections in Ireland. J. Clin. Microbiol. 2010; 48(9): 3288–94. https://doi.org/10.1128/JCM.00434-10
  16. Anis E., Grotto I., Moerman L., Warshavsky B., Slater P.E., Lev B. Mumps outbreak in Israel’s highly vaccinated society: are two doses enough? Epidemiol. Infect. 2012; 140(3): 439–46. https://doi.org/10.1017/S095026881100063X
  17. Otto W., Mankertz A., Santibanez S., Saygili H., Wenzel J., Jilg W., et al. Ongoing outbreak of mumps affecting adolescents and young adults in Bavaria, Germany, August to October 2010. Euro Surveill. 2010; 15(50): 19748.
  18. Echevarría J.E., Castellanos A., Sanz J.C., Pérez C., Palacios G., Martínez de Aragón M.V., et al. Circulation of mumps virus genotypes in Spain from 1996 to 2007. J. Clin. Microbiol. 2010; 48(4): 1245–54. https://doi.org/10.1128/JCM.02386-09
  19. Rivailler P., Hickman C., Rota P. Mumps surveillance in the United States. Available at: https://www.ncbi.nlm.nih.gov/nuccore/KF143768.1
  20. Hiebert J., Severini A. Mumps surveillance in Canada. Available at: https://www.ncbi.nlm.nih.gov/popset/?term=MN911767.1
  21. Hiebert J., Schulz H., Zubach V., Severini A. Mumps surveillance in Canada. Available at: https://www.ncbi.nlm.nih.gov/popset?DbFrom=nuccore&Cmd=Link&LinkName=nuccore_popset&IdsFromResult=1796908945
  22. Hiebert J., Severini A. Mumps surveillance in Canada. Available at: https://www.ncbi.nlm.nih.gov/popset?DbFrom=nuccore&Cmd=Link&LinkName=nuccore_popset&IdsFromResult=1796906304
  23. Dembinski J.L. Mumps virus genotype G strain MuVs/Fredrikstad.NOR/13.20[G] small hydrophobic protein (SH) gene, complete cds. Available at: https://www.ncbi.nlm.nih.gov/nuccore/MT339438.1
  24. Tallo T., Brytting M. Mumps orthorubulavirus small hydrophobic protein (SH) gene, complete cds. Available at: https://www.ncbi.nlm.nih.gov/popset?DbFrom=nuccore&Cmd=Link&LinkName=nuccore_popset&IdsFromResult=1479792882
  25. Dembinski J.L. Mumps virus genotype G strain MuVs/Fredrikstad.NOR/13.20[G] small hydrophobic protein (SH) gene, complete cds. Available at: https://www.ncbi.nlm.nih.gov/nuccore/MT339438.1
  26. Mishra B., Pujhari S.K., Dhiman V., Mahalakshmi P., Bharadwaj A., Pokhrel S., et al. Genotyping and subtyping of mumps virus isolates from the Indian subcontinent. Arch. Virol. 2013; 158(11): 2359–63. https://doi.org/10.1007/s00705-013-1717-4

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2. The phylogenetic tree of mumps virus strains isolated in the RF in 2022 (n = 11, SH-gene fragment 316 nt in length).

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Copyright (c) 2023 Rubalskaia T.S., Erokhov D.V., Zherdeva P.E., Milikhina A.V., Gadzhiewa A.A., Tikhonova N.T.

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