Isolation of a new strain М-2020 of the camelpox virus (Poxviridae: Orthopoxvirus: Camelpox virus) in Republic of Kazakhstan and study of its reproduction in various biological systems

Cover Image

Cite item

Full Text

Abstract

Introduction. This article presents the results of isolation of camel smallpox virus (Poxviridae: Orthopoxvirus: Camelpox virus, CMLPV) and study of its reproductive properties on sensitive biological systems.

Material and methods. The epizootic strain M-96 of the virus as well as its attenuated variants KM-40 and KM-70 obtained by sequential passivation were used in the study. Isolation of the pathogen from suspension of biopsy specimens was performed on cell culture and in embryonated chicken eggs (ECEs). All experiments were performed with the number of replications ensuring obtaining reliable results.

Results. The CMLPV was isolated from the crusts and pox papules of the skin taken from sick camels (Camelus bactrianus) during an outbreak in various districts of the Mangistau region at the end of 2019. The signs of pathogen reproduction on chorio-allantoic membrane (CAM) were observed from 3 passages. The obtained virus caused formation of pathological changes on the CAM in the form of elevated dot or solid white formations separated from the surrounding tissue, with hemorrhagic foci in the center. The reproductive properties of the isolate on sensitive biological systems were determined in comparison with the epizootic CMLPV strain M-96, isolated earlier in the territory of Kazakhstan during the outbreak 23–24 years ago, as well as its attenuated variants. The isolated virus was given the conventional name M-2020.

Discussion. When studied in two sensitive cultivation systems (cell culture and ECEs), strain M-96 and its attenuated variants KM-40, KM-70, which were used in the experiments as a control, demonstrated high infectious activity with titer 4.75-6.75 lg TCID50/cm3, while for the examined isolate M-2020 of CMLPV had the significantly lower values (3.00-4.75 lg TCID50/cm3, p > 0,05).

About the authors

К. D. Zhugunissov

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0003-4238-5116

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

М. А. Mambetaliyev

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0001-6034-6642

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

М. А. Azanbekova

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0002-5807-7604

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

М. К. Kenzhebaeva

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0001-6666-6532

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

S. S. Kilibayev

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0001-9203-2189

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

M. S. Tuyskanova

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan; NJSC «Al-Farabi Kazakh National University»

Author for correspondence.
Email: monica_94@list.ru
ORCID iD: 0000-0001-6565-082X

Moldir S. Tuyskanova, M.Biol., Junior Researcher, Laboratory «Collection of Microorganisms»

080409, Gvardeyskiy vill., Zhambyl region, Korday district; 050040, Almaty

Kazakhstan

A. S. Dzhapasheva

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0002-7414-4635

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

A. D. Omurtay

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0002-9331-5161

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

Sh. T. Tabys

DGE «Research Institute for Biological Safety Problems», Committee of Science of the Ministry of Education and Science of the Republic of Kazakhstan

ORCID iD: 0000-0002-4909-6598

080409, Gvardeyskiy vill., Zhambyl region, Korday district

Kazakhstan

References

  1. Сатбеков Е.С. Получение и испытание на специфическую активность гипериммунной сыворотки против оспы верблюдов. Вестник сельскохозяйственной науки. 1971; (2): 101–4.
  2. Трабаев О. Результаты изучения устойчивости вируса оспы верблюдов в различных условиях внешней среды и при воздействии антибиотиков и дезосредств. В кн.: Труды Алма-Атинского ЗВИ. Том ХХ: Инфекционные и паразитарные заболевания сельскохозяйственных животных. Алма-Ата; 1972: 34–5.
  3. Садыков Р.Г. Культивирование вируса оспы верблюдов на куриных эмбрионах. Вирусные болезни сельскохозяйственных животных. 1970; (1): 1–25.
  4. Булатов Е.А., Мамадалиев С.М., Мамбеталиев М. Изучение основных биофизических характеристик вируса оспы верблюдов из штамма «М-96». Вестник науки Казахского агротехнического университета им. С. Сейфулина. 2009; (4): 225–9.
  5. Булатов Е.А., Мамадалиев С.М., Мамбеталиев М., Битов Н.Т. О циркуляции вируса оспы верблюдов в Мангистауской области Республики Казахстан в скрытой форме. Актуальные вопросы ветеринарной биологии. 2010; (3): 10–3.
  6. Султанкулова К.Т., Зайцев В.Л. Вирус оспы верблюдов: биологические свойства, структура генома и диагностика. Морфология и структура вируса. Алматы; 2017: 37–40.
  7. Сюрин В.Н. Руководство по ветеринарной вирусологии. Москва: Колос; 1966.
  8. Сергеев В.А. Размножение вируса животных в клетках ткани. Москва: Колос; 1966.
  9. Marennikova S.S., Shenkman L.S., Shelukhina E.M., Maltseva N.N. Isolation of camel pox virus and investigation of its properties. Acta virologica. 1974; 18(5): 423–8.
  10. Afonso C.L., Tulman E.R., Lu Z., Zsak L., Sandybaev N.T., Kerembekova Z.U., et al. The genome of camelpox virus. Virology. 2002; 295(1): 1–9. https://doi.org/10.1006/viro.2001.1343
  11. OIE 2019. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2019. Chapter 3.9.2. Camelpox. Paris: World Organisation for Animal Health; 2019: 1665–8.
  12. Reed L.J., Muench H. A simple method of estimating fifty percent endpoints. Am. J. Epidemiol. 1938; 27(3): 493–7. https://doi.org/10.1093/oxfordjournals.aje.a118408
  13. Tantawi H.H., Saban M.S., Reda I.M., Dahaby H.E. Camel pox virus in Egypt. Isolation and characterization. Bull. Epizoot. Dis. Africa. 1974; 22(4): 315–9.
  14. Chauhan R.S., Kaushik R.K. Isolation of camel pox virus in India. Br. Vet. J. 1987; 143(6): 581–2. https://doi.org/10.1016/0007-1935(87)90050-9
  15. Marodam V., Nagendrakumar S.B., Tanwar V.K., Thiagarajan D., Reddy G.S., Tanwar R.K., et al. Isolation and identification of camel pox virus. Indian J. Anim. Sci. 2006; 76: 326–7.
  16. Ramyar H., Hessami M. Isolation, cultivation and characterization of camel pox virus. Arch. Inst. Razi. 1972; 24: 13–21.
  17. Davies F., Mungai J., Shaw T. Characteristics of a Kenyan camelpox virus. J. Hyg. (Lond.). 1975; 75(3): 381–5. https://doi.org/10.1017/s002217240002444x
  18. Gitao C.G., Nyaga P.N. Pathogenicity of Camelpox virus strain from Kenya on Camels (Camelus dromedarius). Bull. Anim. Health Prod. Afr. 1995; 43: 247–51.
  19. Klopries M., Wernery U., Karden O.-R. Characterisation of the camel skin cell line Dubca. Br. Vet. J. 1995; 151(5): 555–65. https://doi.org/10.1016/s0007-1935(05)80026-0
  20. Tantawi H.H., El-Dahaby H., Fanmy L.S. Comparative studies on poxvirus strains isolated from camels. Acta Virol. 1978; 22(6): 451–7.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2022 Zhugunissov К.D., Mambetaliyev М.А., Azanbekova М.А., Kenzhebaeva М.К., Kilibayev S.S., Tuyskanova M.S., Dzhapasheva A.S., Omurtay A.D., Tabys S.T.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Согласие на обработку персональных данных

 

Используя сайт https://journals.rcsi.science, я (далее – «Пользователь» или «Субъект персональных данных») даю согласие на обработку персональных данных на этом сайте (текст Согласия) и на обработку персональных данных с помощью сервиса «Яндекс.Метрика» (текст Согласия).