DNA mapping in the capsid of giant bacteriophage phiEL (Caudovirales: Myoviridae: Elvirus) by analytical electron microscopy
- Authors: Trifonova T.S.1,2, Moiseenko A.V.2,3, Bourkaltseva M.V.4, Shaburova O.V.4, Shaytan A.K.2, Krylov V.N.4, Sokolova O.S.2
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Affiliations:
- FSAEI HE «People’s Friendship University of Russia», Physical, Mathematical, and Natural Sciences Department
- FSBEI HE «Lomonosov Moscow State University», Bioengineering Department, Biological Faculty
- FSBIS «N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences»
- FSBRI «I.I. Mechnikov Research Institute of Vaccines and Sera»
- Issue: Vol 66, No 6 (2021)
- Pages: 434-441
- Section: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/118210
- DOI: https://doi.org/10.36233/0507-4088-80
- ID: 118210
Cite item
Abstract
Introduction. Giant phiKZ-like bacteriophages have a unique protein formation inside the capsid, an inner body (IB) with supercoiled DNA molecule wrapped around it. Standard cryo-electron microscopy (cryo-EM) approaches do not allow to distinguish this structure from the surrounding nucleic acid of the phage. We previously developed an analytical approach to visualize protein-DNA complexes on Escherichia coli bacterial cell slices using the chemical element phosphorus as a marker. In the study presented, we adapted this technique for much smaller objects, namely the capsids of phiKZ-like bacteriophages.
Material and methods. Following electron microscopy techniques were used in the study: analytical (AEM) (electron energy loss spectroscopy, EELS), and cryo-EM (images of samples subjected to low and high dose of electron irradiation were compared).
Results. We studied DNA packaging inside the capsids of giant bacteriophages phiEL from the Myoviridae family that infect Pseudomonas aeruginosa. Phosphorus distribution maps were obtained, showing an asymmetrical arrangement of DNA inside the capsid.
Discussion. We developed and applied an IB imaging technique using a high angle dark-field detector (HAADF) and the STEM-EELS analytical approach. Phosphorus mapping by EELS and cryo-electron microscopy revealed a protein formation as IB within the phage phiEL capsid. The size of IB was estimated using theoretical calculations.
Conclusion. The developed technique can be applied to study the distribution of phosphorus in other DNA- or RNA-containing viruses at relatively low concentrations of the element sought.
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##article.viewOnOriginalSite##About the authors
T. S. Trifonova
FSAEI HE «People’s Friendship University of Russia», Physical, Mathematical, and Natural Sciences Department; FSBEI HE «Lomonosov Moscow State University», Bioengineering Department, Biological Faculty
Email: fake@neicon.ru
ORCID iD: 0000-0003-2042-5244
115419, Moscow, Russia
119234, Moscow, Russia
Russian FederationA. V. Moiseenko
FSBEI HE «Lomonosov Moscow State University», Bioengineering Department, Biological Faculty; FSBIS «N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences»
Email: fake@neicon.ru
ORCID iD: 0000-0003-1112-2356
119234, Moscow, Russia
119234, Moscow, Russia
Russian FederationM. V. Bourkaltseva
FSBRI «I.I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
ORCID iD: 0000-0003-3793-1354
105064, Moscow, Russia
Russian FederationO. V. Shaburova
FSBRI «I.I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
ORCID iD: 0000-0003-0368-3794
105064, Moscow, Russia
Russian FederationA. K. Shaytan
FSBEI HE «Lomonosov Moscow State University», Bioengineering Department, Biological Faculty
Email: fake@neicon.ru
ORCID iD: 0000-0003-0312-938X
119234, Moscow, Russia
Russian FederationV. N. Krylov
FSBRI «I.I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
ORCID iD: 0000-0001-5775-5146
105064, Moscow, Russia
Russian FederationO. S. Sokolova
FSBEI HE «Lomonosov Moscow State University», Bioengineering Department, Biological Faculty
Author for correspondence.
Email: sokolova@mail.bio.msu.ru
ORCID iD: 0000-0003-4678-232X
Olga S. Sokolova, D.Sci. (Biol.), RAS Professor, Professor of Bioengineering Department, Biological Faculty
119234, Moscow, Russia
Russian FederationReferences
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