Flow cytometry evaluation of the rhesus monkey cellular immunity following the Zaire ebolavirus (Filoviridae; Ebolavirus: Zaire ebolavirus) experimental infection

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Abstract

Introduction. The outbreaks of the Zaire ebolavirus (ZE) disease (ZED) that have arisen in the last decade determine the need to study the infection pathogenesis, the formation of specific immunity forming as well as the development of effective preventive and therapeutic means. All stages of fight against the ZED spread require the experimental infection in sensitive laboratory animals, which are rhesus monkeys in case of this disease .

The aim of the study is to evaluate the rhesus monkey cellular immunity following the ZE experimental infection by the means of flow cytometry (cytofluorimetry).

Material and methods. Male rhesus monkeys were intramuscularly infected by the dose of 15 LD50 (dose of the pathogen that causes 50% mortality of infected animals) of the ZE, the Zaire strain (ZEBOV). Levels of 18 peripheral blood lymphocyte populations of the animals before the ZE experimental infection and at the terminal stage of the disease were assessed using flow cytometry.

Results and discussion. The certain changes in the levels of the lymphocyte populations were observed following infection, indicating simultaneous activation and suppression of the immune system during ZED. The increase in content was observed for T-lymphocytes, T-helper and cytotoxic T-lymphocytes expressing the corresponding markers of early activation. The decrease was recorded for T-lymphocytes and double-positive T-lymphocytes expressing corresponding markers of late activation, as well as natural killer cells expressing CD8 (p < 0.05).

Conclusion. For the first time in the Russian Federation, the rhesus monkey cellular immunity before and after the ZE experimental infection was assessed using flow cytometry.

About the authors

G. V. Borisevich

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

Author for correspondence.
Email: 48cnii@mil.ru
ORCID iD: 0000-0002-0843-9427

Galina V. Borisevich, Ph.D. (Biol.), Senior Researcher of the Department

141306, Sergiev Posad-6, Russia

Russian Federation

S. L. Kirillova

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0003-1245-9225

141306, Sergiev Posad-6, Russia

Russian Federation

I. V. Shatokhina

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-9503-5120

141306, Sergiev Posad-6, Russia

Russian Federation

V. N. Lebedev

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-6552-4599

141306, Sergiev Posad-6, Russia

Russian Federation

N. V. Shagarova

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0001-9523-8676

141306, Sergiev Posad-6, Russia

Russian Federation

S. I. Syromyatnikova

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-1490-9448

141306, Sergiev Posad-6, Russia

Russian Federation

A. F. Andrus

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-7430-9401

141306, Sergiev Posad-6, Russia

Russian Federation

E. A. Koval’chuk

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-7279-196X

141306, Sergiev Posad-6, Russia

Russian Federation

V. B. Kirillov

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0003-2916-0668

141306, Sergiev Posad-6, Russia

Russian Federation

M. L. Bespalov

LLC «MedisСoM»

ORCID iD: 0000-0003-3220-6153

127254, Moscow, Russia

Russian Federation

A. A. Petrov

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-9714-2085

141306, Sergiev Posad-6, Russia

Russian Federation

A. V. Koval’chuk

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-9681-9891

141306, Sergiev Posad-6, Russia

Russian Federation

V. B. Pantyukhov

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-1313-2059

141306, Sergiev Posad-6, Russia

Russian Federation

D. A. Kutayev

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0001-9009-4909

141306, Sergiev Posad-6, Russia

Russian Federation

S. V. Borisevich

FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0002-6742-3919

141306, Sergiev Posad-6, Russia

Russian Federation

S. L. Kuznetsov

Directorate of the Chief of the Radiation, Chemical, and Biological Defence Troops of the Armed Forces of the Ministry of Defence of the Russian Federation

ORCID iD: 0000-0003-2705-8774

119160, Moscow, Russia

Russian Federation

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Copyright (c) 2021 Borisevich G.V., Kirillova S.L., Shatokhina I.V., Lebedev V.N., Shagarova N.V., Syromyatnikova S.I., Andrus A.F., Koval’chuk E.A., Kirillov V.B., Bespalov M.L., Petrov A.A., Koval’chuk A.V., Pantyukhov V.B., Kutayev D.A., Borisevich S.V., Kuznetsov S.L.

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