Vaccination with virus-like particles containing hemagglutinin protects the lungs of mice with postifluenza bacterial pneumonia: virological, microbiological and clinical data
- 作者: Falynskova I.N.1, Egorov A.Y.1,2, Poddubikov A.V.1, Vartanova N.O.1, Kartashova N.P.1, Glubokova E.A.1, Mkhitarov V.A.3, Dzhalilova D.S.3, Makarova O.V.3, Leneva I.A.1
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隶属关系:
- I.I. Mechnikov Research Institute of Vaccines and Sera
- Smorodintsev Research Institute of Influenza
- Research Institute of Human Morphology
- 期: 卷 65, 编号 3 (2020)
- 页面: 150-158
- 栏目: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/118137
- DOI: https://doi.org/10.36233/0507-4088-2020-65-3-150-158
- ID: 118137
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Introduction. Influenza is a severe viral disease, a frequent complication of which is a secondary bacterial pneumonia. Influenza vaccines prevent secondary bacterial complications. Virus-like particles are one of the promising areas for the development of new vaccines.
The aim of this work is to study the correlation of the pathomorphological characteristics of the lungs with clinical, virological, and microbiological markers of the disease at vaccination with virus-like particles (VLPs), containing hemagglutinin (HA) of influenza virus (HA-Gag-VLPs) in a murine model of secondary bacterial pneumonia induced by S. pneumoniae after influenza infection.
Material and methods. BALB/c mice were vaccinated with VLPs containing influenza HA. After 21 days, mice were infected with two strains of influenza viruses, homologous and non-homologous, and 5 days after viral infection, were infected with S. pneumoniae. The vaccination effect was evaluated by morphological, virological (titer of the virus in the lungs) and microbiological (titer of bacteria in the lungs) data, and was confirmed by clinical data (survival, change in body weight).
Results. Immunization with HA-Gag-VLPs, followed by infection with a homologous influenza virus and S. pneumoniae, reduced the area of foci of inflammation, inhibited the replication of the virus and bacteria in the lungs, and also protected animals from death and reduced their weight loss. Immunization with HA-Gag-VLPs upon infection with a heterologous strain and S. pneumoniae did not affect these criteria.
Conclusion. The immunization with HA-Gag-VLPs prevented the viral replication, providing a reduction of S. pneumoniae titer and the degree of lung damage, protecting animals from the disease in a murine model of secondary bacterial pneumonia, induced by S. pneumoniae, after influenza infection with homologous strain of the virus.
作者简介
I. Falynskova
I.I. Mechnikov Research Institute of Vaccines and Sera
编辑信件的主要联系方式.
Email: falynskova@mail.ru
ORCID iD: 0000-0001-9836-9620
Irina N. Falynskova, researcher of Laboratory experimental virology
Moscow, 105064
俄罗斯联邦A. Egorov
I.I. Mechnikov Research Institute of Vaccines and Sera; Smorodintsev Research Institute of Influenza
Email: fake@neicon.ru
ORCID iD: 0000-0003-2068-3745
Andrej Yu. Egorov
Moscow, 105064
Saint Petersburg, 197022
俄罗斯联邦A. Poddubikov
I.I. Mechnikov Research Institute of Vaccines and Sera
Email: fake@neicon.ru
ORCID iD: 0000-0001-8962-4765
Alexander V. Poddubikov
Moscow, 105064
俄罗斯联邦N. Vartanova
I.I. Mechnikov Research Institute of Vaccines and Sera
Email: fake@neicon.ru
ORCID iD: 0000-0002-6372-9910
Nune O. Vartanova
Moscow, 105064
俄罗斯联邦N. Kartashova
I.I. Mechnikov Research Institute of Vaccines and Sera
Email: fake@neicon.ru
ORCID iD: 0000-0003-2096-5080
Nadezhda P. Kartashova
Moscow, 105064
俄罗斯联邦E. Glubokova
I.I. Mechnikov Research Institute of Vaccines and Sera
Email: fake@neicon.ru
ORCID iD: 0000-0002-5925-9733
Ekaterina A. Glubokova
Moscow, 105064
俄罗斯联邦V. Mkhitarov
Research Institute of Human Morphology
Email: fake@neicon.ru
ORCID iD: 0000-0002-4427-1991
Vladimir A. Mkhitarov
Moscow, 117418
俄罗斯联邦D. Dzhalilova
Research Institute of Human Morphology
Email: fake@neicon.ru
ORCID iD: 0000-0002-1337-7160
Dzhuliya S. Dzhalilova
Moscow, 117418
俄罗斯联邦O. Makarova
Research Institute of Human Morphology
Email: fake@neicon.ru
ORCID iD: 0000-0001-8581-107X
Olga V. Makarova
Moscow, 117418
俄罗斯联邦I. Leneva
I.I. Mechnikov Research Institute of Vaccines and Sera
Email: fake@neicon.ru
ORCID iD: 0000-0002-7755-2714
Irina A. Leneva
Moscow, 105064
俄罗斯联邦参考
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