Comparative molecular and genetic characterization of rabies viruses (Rabies lyssavirus, Lyssavirus, Rhabdoviridae) circulated in the Russian Federation in 1985–2016
- Authors: Zaykova O.N.1,2, Grebennikova T.V.1,2, Losich M.A.1, Elakov A.L.1, Gulyukin A.M.3, Metlin A.E.4
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Affiliations:
- National Research Center for Epidemiology and Microbiology named after honorary academician N.F. Gamaleya
- Peoples’ Friendship University of Russia
- All-Russian Scientific and Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences
- All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
- Issue: Vol 65, No 1 (2020)
- Pages: 41-48
- Section: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/118041
- DOI: https://doi.org/10.36233/0507-4088-2020-65-1-41-48
- ID: 118041
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Abstract
Introduction. Rabies caused by the neurotropic virus of the genus Lyssavirus, Rhabdoviridae family, which infects all warm-blooded vertebrates including human beings. The homology level of the amino acid sequences for Lyssaviruses nucleoprotein reaches 78–93%.
Aim – study the genetic diversity and molecular epidemiology of Lyssaviruses circulated in the Russian Federation in 1985–2016.
Material and methods. 54 isolates of rabies virus isolated from animals, and 2 isolates from humans, 4 vaccine strains of rabies virus: RV-97, ERA, Shchelkovo 51, ERAG333 used in phylogenetic study. Phylogenetic analysis was performed using Genbank data on genome fragments of 73 rabies virus isolates and 9 EBLV-1 isolates. DNASTAR V.3.12, Bio Edit 7.0.4.1 and MEGA v.10.0.5, Primer Premier 5 programs have been used.
Results. Comparative molecular genetic analysis of genomes fragments of 130 Lissaviruses, isolated on the territory of the RF, Ukraine in 1985-2016, vaccine strains of rabies virus, showed their distribution by geographical feature. Comparison of the nucleoprotein fragments of the rabies virus isolates with vaccine strains revealed 4 marker mutations: V56I (Eurasian group), L/V95W (Central group), D101N/S/T, and N/G106D. Phylogenetic analysis of the isolate «Juli», isolated from a human bitten by a bat proved his belonging to the European Bat lyssavirus-1a.
Discussion. Study of the molecular epidemiology of rabies within the Russian Federation allows for the genotyping of the viruses and helps to study the hidden mechanisms of rabies infection in animal and human populations, and to characterize vaccine strains, including during oral vaccination.
Conclusion. Further study of the molecular epidemiology of rabies within the Russian Federation and the countries bordering it is important.
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##article.viewOnOriginalSite##About the authors
O. N. Zaykova
National Research Center for Epidemiology and Microbiology named after honorary academician N.F. Gamaleya; Peoples’ Friendship University of Russia
Author for correspondence.
Email: zaykova_o_n@mail.ru
ORCID iD: 0000-0003-4708-2069
Olga N. Zaykova, Researcher at the Laboratory of Molecular Diagnostics.
Moscow, 123098
Moscow, 117198
T. V. Grebennikova
National Research Center for Epidemiology and Microbiology named after honorary academician N.F. Gamaleya; Peoples’ Friendship University of Russia
Email: fake@neicon.ru
ORCID iD: 0000-0002-6141-9361
Moscow, 123098
Moscow, 117198 Russian Federation
M. A. Losich
National Research Center for Epidemiology and Microbiology named after honorary academician N.F. Gamaleya
Email: fake@neicon.ru
ORCID iD: 0000-0002-5618-1918
Moscow, 123098 Russian Federation
A. L. Elakov
National Research Center for Epidemiology and Microbiology named after honorary academician N.F. Gamaleya
Email: fake@neicon.ru
ORCID iD: 0000-0001-5798-6518
Moscow, 123098 Russian Federation
A. M. Gulyukin
All-Russian Scientific and Research Institute of Experimental Veterinary Medicine named after K.I. Scriabin and Ya.R. Kovalenko of the Russian Academy of Sciences
Email: fake@neicon.ru
ORCID iD: 0000-0003-2160-4770
Moscow, 109428 Russian Federation
A. E. Metlin
All-Russian State Center for Quality and Standardization of Medicines for Animals and Feed
Email: fake@neicon.ru
ORCID iD: 0000-0002-4283-0171
Moscow, 123022 Russian Federation
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