ADAPTATION OF THE CORN EARWORM SINGLE NUCLEOCAPCIDE NUCLEOPOLYHEDROVIRUS (HELICOVERPA ZEA SNPV) FOR THE CONTROL OF THE COTTON BOLLWORM (HELICOVERPA ARMIGERA) POPULATION
- 作者: Kolosov A.V.1, Ternovoy V.A.1, Shvalov A.N.1, Moiseeva A.A.1, Safatov A.S.1, Mikheev V.N.1
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隶属关系:
- State Research Center of Virology and Biotechnology «Vector»
- 期: 卷 62, 编号 3 (2017)
- 页面: 134-137
- 栏目: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/117945
- DOI: https://doi.org/10.18821/0507-4088-2017-62-3-134-137
- ID: 117945
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详细
Helicoverpa zea (Boddie, 1850) (Hz) single nucleocapcide nucleopolyhedrovirus (SNPV) was adapted to the cotton bollworm (Helicoverpa armigera, (Hübner, 1805) (Ha)) by five blind passages on larvae. The full genomic sequence of the resulting strain HS-18 has been determined (GenBank acc. №: KJ004000.1). Biological activity of the HS-18 strain is higher than the activity of all other Russian strains of NPV, as far as cotton bollworm strain HearSNPV-G4. HS-18-infected caterpillars at the 3-rd and 4-th ages died much faster than those infected with HearSNPV-G4 strain. A major difference of HS-18 genome is an 18 bp repeat in the RING-finger ORF that confirms high variability of this region. Three other insertions and seven base substitutions were observed earlier, while six base substitutions are new. Mutations are located at ORF42, lef-9, ORF58, VP39, PIF-4, P48, SOD, ORF111, ORF129 and ORF138 genes. Among all nucleotide mutation only one is synonymous. Thus we suppose the selective pressure to the virus. The resulting strain HS-18 is recommended as a biopesticide for controlling the number of cotton bollworm in cotton fields.
作者简介
A. Kolosov
State Research Center of Virology and Biotechnology «Vector»
编辑信件的主要联系方式.
Email: kolosov_av@vector.nsc.ru
俄罗斯联邦
V. Ternovoy
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
俄罗斯联邦
A. Shvalov
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
俄罗斯联邦
A. Moiseeva
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
俄罗斯联邦
A. Safatov
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
俄罗斯联邦
V. Mikheev
State Research Center of Virology and Biotechnology «Vector»
Email: noemail@neicon.ru
俄罗斯联邦
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