Email this article Prostranstvennaya model' organizatsii khromatina v yadre biologicheskoy kletki po dannym malouglovogo rasseyaniya
- Authors: Yashina E.G.1,2, Varfolomeeva E.Y.1, Pantina R.A.1, Bayramukov V.Y.1, Kovalev R.A.1, Fedorova N.D.1, Pshenichnyy K.A.1, Gorshkova Y.E.3, Grigor'ev S.V.1,2
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Affiliations:
- Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute
- St. Petersburg State University
- Joint Institute for Nuclear Research
- Issue: Vol 118, No 9-10 (11) (2023)
- Pages: 776-781
- Section: Articles
- URL: https://journals.rcsi.science/0370-274X/article/view/247005
- DOI: https://doi.org/10.31857/S1234567823220123
- EDN: https://elibrary.ru/PFTLYR
- ID: 247005
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Abstract
Small-angle neutron and X-ray scattering data for HeLa nuclei with the normal and suppressed transcription activities are reported. Small-angle neutron scattering data demonstrate the presence of a bifractal structure inside a nucleus. The logarithmic fractal structure is observed in the range from the size of the nucleus to several hundreds of nanometer, whereas the volume fractal structure exists at smaller scales down to a nucleosome structure. Small-angle X-ray scattering data show that the presence of the volume fractal structure correlates with the transcription activity of a cell. In view of the successful description of chromatin by the fractal globule model (Hi–C method data), a scenario for the formation of the bifractal structure inside the nucleus has been proposed. A system of transport channels (logarithmic fractal) is located inside close-packed chromatin, whereas active chromatin is localized near transport channels or inside them and forms volume fractal structures due to the transcription activity. Thus, the logarithmic fractal structure ensures the uniform distribution of voids at various scales, which is potentially necessary for the transcription and transport of substances inside the nucleus, whereas the volume fractal structure is due to the transcription activity of the cell.
About the authors
E. G. Yashina
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute; St. Petersburg State University
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia; 198504, St. Petersburg, Russia
E. Yu. Varfolomeeva
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia
R. A. Pantina
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia
V. Yu. Bayramukov
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia
R. A. Kovalev
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia
N. D. Fedorova
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia
K. A. Pshenichnyy
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia
Yu. E. Gorshkova
Joint Institute for Nuclear Research
Email: yashina_91@inbox.ru
141980, Dubna, Moscow region, Russia
S. V. Grigor'ev
Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute; St. Petersburg State University
Author for correspondence.
Email: yashina_91@inbox.ru
188300, Gatchina, Leningrad region, Russia; 198504, St. Petersburg, Russia
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