Nanoporous Glass with Controlled Pore Size for High-Efficiency Synthesis of Oligonucleotides

Abstract

The results of work on the development of powder nanoporous glass (NPG) with controlled pore size for solid-phase synthesis of oligonucleotides with heightened product yield are presented. NPG with a narrow pore-size distribution was obtained by varying the parameters of the heat and chemical treatment of sodium-borosilicate glasses. It was found that the functional charge of the material is equal to 72 μmol/g. It is shown that using the developed NPG in the synthesis of long-chain oligonucleotides increases the product yield more than seven-fold compared with the analogous material offered on the market.