Comparative study on the use of mineral oil in the embryological stage of assisted reproductive technology programs

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

Objective: To investigate the effect of mineral oil type on the development of human embryos during in vitro culture in the context of in vitro fertilization (IVF) programs.

Materials and methods: This study involved zygotes/sibling embryos (1198 embryos) obtained from 84 patients undergoing IVF treatment at the AltraVita Human Reproduction Clinic. After fertilization, the sibling embryos of each patient were cultured for 5–6 days in microdrops of medium, either under a layer of mineral oil or under a layer of paraffin oil. The parameters analyzed included fertilization and embryo cleavage rates, blastocyst formation rates, good-quality blastocyst formation rates, embryo implantation rates, and clinical pregnancy rates.

Results: The group of embryos cultured with paraffin oil showed a significantly higher total number of blastocysts and a higher rate of good-quality blastocyst formation compared to the group of embryos cultured under a layer of mineral oil. Fertilization and embryo cleavage rates, as well as implantation and clinical pregnancy rates, were not statistically significantly different between the two groups.

Conclusion: Coating droplets with medium using paraffin oil during in vitro embryo culture improves the efficiency of the embryological stage compared with the use of mineral oil for the same purpose.

作者简介

Nadezhda Skorlupkina

AltraVita Human Reproduction Clinic, IVF CENTER LLC

编辑信件的主要联系方式.
Email: nadezhdaskor@yandex.ru

Senior Embryologist

俄罗斯联邦, Moscow

Valentina Apryshko

AltraVita Human Reproduction Clinic, IVF CENTER LLC; Lomonosov Moscow State University

Email: supermycolog@mail.ru

PhD, Head of Embryology Department; Researcher at the Faculty of Biology

俄罗斯联邦, Moscow; Moscow

Elena Lebedeva

AltraVita Human Reproduction Clinic, IVF CENTER LLC

Email: lebedester@gmail.com

PhD, Coordinator of Medical Research

俄罗斯联邦, Moscow

Konstantin Kirienko

AltraVita Human Reproduction Clinic, IVF CENTER LLC

Email: kkiriyenko@rambler.ru
ORCID iD: 0000-0001-8713-6231

PhD, Leading Embryologist

俄罗斯联邦, Moscow

Anna Mironova

AltraVita Human Reproduction Clinic, IVF CENTER LLC

Email: agm90@mail.ru

Leading Embryologist

俄罗斯联邦, Moscow

Sergey Yakovenko

AltraVita Human Reproduction Clinic, IVF CENTER LLC; Lomonosov Moscow State University

Email: altravita@mail.ru

PhD, CEO; Researcher at Biophisics Department

俄罗斯联邦, Moscow; Moscow

参考

  1. Fukui Y., Lee E.S., Araki N. Effect of medium renewal during culture in two different culture systems on development to blastocysts from in vitro produced early bovine embryos. J. Anim. Sci. 1996; 74(11): 2752-8. https://dx.doi.org/10.2527/1996.74112752x.
  2. Morbeck D.E., Khan Z., Barnidge D.R., Walker D.L. Washing mineral oil reduces contaminants and embryotoxicity. Fertil. Steril. 2010; 94(7): 2747-52. https://dx.doi.org/10.1016/j.fertnstert.2010.03.067.
  3. Ebrahimi M., Mara L., Parham A., Dattena M. Mineral oil for in vitro embryo production: what we should know? Arch. Razi Inst. 2022; 77(4): 1325-30. https://dx.doi.org/10.22092/ARI.2022.358955.2343.
  4. Scarica C., Monaco A., Borini A., Pontemezzo E., Bonanni V., De Santis L. et al.; SIERR, Società Italiana di Embriologia Riproduzione e Ricerca. Use of mineral oil in IVF culture systems: physico-chemical aspects, management, and safety. J. Assist. Reprod. Genet. 2022; 39(4): 883-92. https://dx.doi.org/10.1007/s10815-022-02479-z.
  5. Yoon J., Yoon S., Ko Y., Lim J. P-262: Effect of filtration of mineral oil on the mouse embryonic development in vitro. Fertil. Steril. 2006; 86(3): 232. https://dx.doi.org/10.1016/S0015-0282(06)03381-4.
  6. Labied S., Jouan C., Wenders F., Ravet S., Gaspard O., Thonon F. et al. Comparison between paraffin and mineral oil covering on early human embryo culture: a prospective randomized study. Syst. Biol. Reprod. Med. 2019; 65(1): 81-6. https://dx.doi.org/10.1080/19396368.2018.1492645.
  7. Gardner D.K., Reed L., Linck D., Sheehan C., Lane M. Quality control in human in vitro fertilization. Semin. Reprod. Med. 2005; 23(4): 319-24. https://dx.doi.org/10.1055/s-2005-923389.
  8. Mantikou E., Youssef M.A., van Wely M., van der Veen F., Al-Inany H.G., Repping S. et al. Embryo culture media and IVF/ICSI success rates: a systematic review. Hum. Reprod. Update. 2013; 19(3): 210-20. https://dx.doi.org/10.1093/humupd/dms061.
  9. Mestres E., Matia-Algué Q., Villamar A., Casals A., Acacio M., García-Jiménez M. et al. Characterization and comparison of commercial oils used for human embryo culture. Hum. Reprod. 2022; 37(2): 212-25. https://dx.doi.org/10.1093/humrep/deab245.
  10. Sifer C., Pont J.C., Porcher R., Martin-Pont B., Benzacken B., Wolf J.P. A prospective randomized study to compare four different mineral oils used to culture human embryos in IVF/ICSI treatments. Eur. J. Obstet. Gynecol. Reprod. Biol. 2009; 147(1): 52-6. https://dx.doi.org/10.1016/ j.ejogrb.2009.06.023.
  11. Morbeck D.E., Leonard P.H. Culture systems: mineral oil overlay. Methods Mol. Biol. 2012; 912: 325-31. https://dx.doi.org/10.1007/ 978-1-61779-971-6_18.
  12. Kane M.T. Culture media and culture of early embryos. Theriogenology. 1987; 27(1): 49-57. https://dx.doi.org/10.1016/0093-691X(87)90069-0.
  13. Van Soom A., Mahmoudzadeh A.R., Christophe A., Ysebaert M.T., de Kruif A. Silicone oil used in microdrop culture can affect bovine embryonic development and freezability. Reprod. Domest. Anim. 2001; 36(3-4): 169-76. https:// dx.doi.org/10.1046/j.1439-0531.2001.00281.x.
##common.cookie##