A New Peptide from the Venom of the Madagascar Cat-Eyed Snake Madagascarophis colubrinus Blocks Nicotinic Acetylcholine Receptor

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In screening the venoms of various snake species, we found that the venom of the Madagascar cat-eyed snake Madagascarophis colubrinus competes with α-bungarotoxin for binding to the nicotinic acetylcholine receptor from Torpedo californica. Using liquid chromatography, a peptide, called macoluxin and inhibiting the binding of the toxin to the receptor, was isolated from the venom. The amino acid sequence of this 23-amino acid peptide was determined by automatic Edman degradation. Comparison with amino acid sequences of known proteins showed that the macoluxin sequence is homologous to the α-helical region of the sequence of snake venom metalloproteinases. The peptide was synthesized by solid-phase peptide synthesis, and the study of its biological activity showed that it inhibits the binding of α-bungarotoxin to the Torpedo receptor with an IC50 of 47 μM. Macoluxin also reversibly inhibited acetylcholine-induced currents in the muscle-type nicotinic acetylcholine receptor. This is the first data on the presence in the venom of rear fanged snakes of a peptide that can inhibit the nicotinic acetylcholine receptor.

作者简介

E. Kryukova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: utkin@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

D. Ivanov

FKOO “Labkorp”

Email: utkin@ibch.ru
Russia, 107045, Moscow, ul. Trubnaya 12

N. Kopylova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: utkin@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

V. Starkov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: utkin@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

T. Andreeva

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: utkin@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

I. Ivanov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: utkin@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

V. Tsetlin

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

Email: utkin@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

Yu. Utkin

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences

编辑信件的主要联系方式.
Email: utkin@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

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版权所有 © Е.В. Крюкова, Д.А. Иванов, Н.В. Копылова, В.Г. Старков, Т.В. Андреева, И.А. Иванов, В.И. Цетлин, Ю.Н. Уткин, 2023

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