Three-Dimensional Structure of Fab Fragment of Monoclonal Antibody LNKB-2 Complexed with Antigenic Nonaptide from Human Interleukin-2
- 作者: Goryacheva E.1, Artemyev I.1, Pletnevа N.1, Pletnev V.1
-
隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- 期: 卷 49, 编号 3 (2023)
- 页面: 285-290
- 栏目: Articles
- URL: https://journals.rcsi.science/0132-3423/article/view/139151
- DOI: https://doi.org/10.31857/S0132342323010098
- EDN: https://elibrary.ru/GFDHZS
- ID: 139151
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
订阅存取
详细
The three-dimensional structure of the antigen-binding fragment (Fab) of the monoclonal antibody LNKB-2 in complex with the synthetic antigenic nonapeptide of human interleukin-2 (IL-2; Lys-Pro-Leu-Glu-Glu-Val-Leu-Asn-Leu-O) was determined by X-ray method at a resolution of 2.6 Å in crystal space group P212121. The peptide adopts a somewhat distorted α-helical conformation, close to that of fragment 64–72 of the IL-2 antigen. Four out of the six hypervariable loops in the antigen-binding site of the Fab fragment are involved in nonapeptide association through hydrogen bonding, salt bridge formation, and hydrophobic interactions. Moreover, Tyr residues of an antibody play an important role in antigen-antibody recognition.
作者简介
E. Goryacheva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
编辑信件的主要联系方式.
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
I. Artemyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
N. Pletnevа
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
V. Pletnev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
参考
- Sela-Culang I., Kunik V., Ofran Y. // Front. Immunol. 2013. V. 4. P. 302. https://doi.org/10.3389/fimmu.2013.00302
- Burkovitz A., Leiderman O., Sela-Culang I., Byk G., Ofran Y. // J. Immunol. 2013. V. 190. P. 2327–2334. https://doi.org/10.4049/jimmunol.1200757
- Kapingidza A.B., Kowal K., Chruszcz M. // Subcell. Biochem. 2020. V. 94. P. 465–497. https://doi.org/10.1007/978-3-030-41769-7_19
- King M.T., Brooks C.L. // Methods Mol. Biol. 2018. V. 1785. P. 13–27. https://doi.org/10.1007/978-1-4939-7841-0_2
- Smith K.A. // Science. 1988. V. 240. P. 1169–1176. https://doi.org/10.1126/science.3131876
- Smith K.A. // Curr. Opin. Immunol. 1992. V. 4. P. 271–276. https://doi.org/10.1016/0952-7915(92)90076-q
- Lunev V.E., Lukin Yu.V., Kazannykh N.V., Belyaev S.V., Zubov V.P., Nesmeyanov V.A. // Biomed. Sci. 1990. V. 1. P. 68–72.
- Фокин A.В., Афонин П.В., Михайлова И.Ю., Цыганник И.Н., Мареева Т.Ю., Несмеянов B.А., Пэнгборн В., Ли Н., Дюэкс В., Сижак Е., Плетнев В.З. // Биоорг. химия. 2000. Т. 26. С. 571–578. [Fokin A.V., Afonin P.V., Mikhailova I.Yu., Tsygannik I.N., Mareeva T.Yu., Nesmeyanov V.A., Pangborn W., Lee N., Duax W., Ciszak E., Pletnev V.Z. // Russ. J. Bioorg. Chem. 2000. V. 39. P. 512–519.] https://doi.org/10.1007/BF02758622
- Плетнев В.З., Горячева Е.А., Цыганник И.Н., Несмеянов В.А., Плетнев С.В., Пэнгборн В., Дюэкс В. // Биоорг. химия. 2004. Т. 30. С. 466–469. [Pletnev V.Z., Goryacheva E.A., Tsygannik I.N., Nesmeyanov V.A., Pletnev S.V., Pangborn W., Daux W. // Russ. J. Bioorg. Chem. 2004. V. 30. P. 417–420.] https://doi.org/10.1023/b:rubi.0000043783.73562.ef
- Afonin P.V., Fokin A.V., Tsygannik I.N., Mikhailova I.Yu., Onoprienko L.V., Mikhaleva I.I., Ivanov V.T., Mareeva T.Yu., Nesmeyanov V.A., Li N., Pangborn W.A., Duax W.L., Pletnev V.Z. // Protein Sci. 2001. V. 10. P. 1514–1521. https://doi.org/10.1110/ps.3101
- Kabat E.A., Wu T.T., Perry H.M., Gottesman K.S., Foeller C. // Sequences of Proteins of Immunological Interest, 5th ed. U.S. Department of Health and Human Services, Public Health Service, National Institutes of Health, Bethesda, 1991.
- Оноприенко Л.В., Михалева И.И., Иванов В.Т., Войтенков Б.О., Окулов В.Б. // Биоорг. химия. 1996. Т. 22. С. 180–190. [Onoprienko L.V., Mikhaleva I.I., Ivanov V.T., Voitenkov B.O., Okulov V.B. // Russ. J. Bioorg. Chem. 1996. V. 22. P. 156–165.]
- Vagin A., Teplyakov A. // Acta Crystallogr. D Biol. Crystallogr. 2010. V. 66. P. 22–25. https://doi.org/10.1107/S0907444909042589
- Murshudov G.N., Skubák P., Lebedev A.A., Pannu N.S., Steiner R.A., Nicholls R.A., Winn M.D., Long F., Vagin A.A. // Acta Crystallogr. D Biol. Crystallogr. 2011. V. 67. P. 355–367. https://doi.org/10.1107/S0907444911001314
- Emsley P., Lohkamp B., Scott W.G., Cowtan K. // Acta Crystallogr. D Biol. Crystallogr. 2010. V. 66. P. 486–501. https://doi.org/10.1107/S0907444910007493
