Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis

R. A. Miftakhov; Мифтахов Р. А.; T. V. Nasedkina; Наседкина Т. В.; A. S. Zasedatelev; Заседателев А. С.; V. E. Shershov; Шершов В. Е.; V. E. Kuznetsova; Кузнецова В. Е.; A. I. Levashova; Левашова А. И.; S. A. Lapa; Лапа С. А.; V. A. Vasiliskov; Василисков В. А.; A. Yu. Ikonnikova; Иконникова А. Ю.; A. V. Chudinov; Чудинов А. В.

doi:10.31857/S0132342323050044

Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis

Autores: Miftakhov R.¹, Nasedkina T.¹, Zasedatelev A.¹, Shershov V.¹, Kuznetsova V.¹, Levashova A.¹, Lapa S.¹, Vasiliskov V.¹, Ikonnikova A.¹, Chudinov A.¹
Afiliações:
1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Edição: Volume 49, Nº 6 (2023)
Páginas: 641-648
Seção: ПИСЬМА РЕДАКТОРУ
URL: https://journals.rcsi.science/0132-3423/article/view/148163
DOI: https://doi.org/10.31857/S0132342323050044
EDN: https://elibrary.ru/ARRVXQ
ID: 148163

Citar

Texto integral

Acesso aberto
Acesso é fechado

Acesso está concedido
Acesso é fechado

Somente assinantes

Resumo
Sobre autores
Bibliografia
Arquivos suplementares
Estatísticas

Resumo

A method for manufacturing biochips by photolithography with hydrogel cells made of brush copolymers based on acrylic acid and acrylamide, fixed at one end on the surface of a polymer substrate has been developed. Hydrogel cells with reactive carboxyl groups were used for covalent immobilization of oligonucleotide probes. The effectiveness of the method was demonstrated in the hybridization analysis of DNA by targets of different lengths of the sequence site 7 of the exon of the human ABO gene.

Palavras-chave

biochips, brush polymers, immobilization of oligonucleotides, DNA hybridization analysis

Bibliografia

Rubina A.Y., Pan’kov S.V., Dementieva E.I., Pen’kov D.N., Butygin A.V., Vasiliskov V.A., Chudinov A.V., Mikheikin A.L., Mikhailovich V.M., Mirzabekov A.D. // Anal. Biochem. 2004. V. 325. P. 92–106. https://doi.org/10.1016/j.ab.2003.10.010
Gryadunov D., Dementieva E., Mikhailovich V., Nasedkina T., Rubina A., Savvateeva E., Fesenko E., Chudinov A., Zimenkov D., Kolchinsky A., Zasedatelev A. // Exp. Rev. Mol. Diagn. 2011. V. 11. P. 839–853. https://doi.org/10.1586/ERM.11.73
Naidu C.K., Suneetha Y. // Trop. J. Pharm. Res. 2012. V. 11. P. 153–164. https://doi.org/10.4314/tjpr.v11i1.20
Beaudet A.L., Belmont J.W. // Annu. Rev. Med. 2008. V. 59. P. 113–129. https://doi.org/10.1146/annurev.med.59.012907.101800
Brittain W.J., Brandstetter T., Prucker O., Rühe J. // ACS Appl. Mat. Int. 2019. V. 11. P. 39397–39409. https://doi.org/10.1021/acsami.9b06838
Золотов А.М., Мифтахов Р.А., Иконникова А.Ю., Лапа С.А., Кузнецова В.Е., Василисков В.А., Шершов В.Е., Заседателев А.С., Наседкина Т.В., Чудинов А.В. // Биоорг. химия. 2022. Т. 48. С. 599–605. [Zolotov A.M., Miftakhov R.A., Ikonnikova A.Y., Lapa S.A., Kuznetsova V.E., Vasiliskov V.A., Shershov V.E., Zasedatelev A.S., Nasedkina T.V., Chudinov A.V. // Russ. J. Bioorg. Chem. 2022. V. 48. Р. 858–863.] https://doi.org/10.1134/S1068162022040203
Shchepinov M.S., Case-Green S.C., Southern E.M. // Nucleic Acids Res. 1997. V. 25. P. 1155–1161. https://doi.org/10.1093/nar/25.6.1155
Halperin A., Buhot A., Zhulina E.B. // Langmuir. 2006. V. 22. P. 11290–11304. https://doi.org/10.1021/la0616606
Zubtsov D.A., Savvateeva E.N., Rubina A.Yu., Pan’kov S.V., Konovalova E.V., Moiseeva O.V., Chechetkin V.R., Zasedatelev A.S. // Anal. Biochem. 2007. V. 368. P. 205–213. https://doi.org/10.1016/j.ab.2007.04.040
Rohr T., Ogletree D.F., Svec F., Fréchet L.M.J. // Adv. Funct. Mat. 2003. V. 13. P. 264–270. https://doi.org/10.1002/adfm.200304229
Ma H., Davis R.H., Bowman C.N. // Macromolecules. 2000. V. 33. P. 331–335. https://doi.org/10.1021/ma990821s
Barbey R., Lavanant L., Paripovic D., Schüwer N., Sugnaux C., Tugulu S., Klok H.-A. // Chem. Rev. 2009. V. 109. P. 5437–5527. https://doi.org/10.1021/cr900045a
Miftakhov R.A., Lapa S.A., Shershov V.E., Zasedateleva O.A., Guseinov T.O., Spitsyn M.A., Kuznetsova V.E., Mamaev D.D., Lysov Yu.P., Barsky V.E., Timofeev E.N., Zasedatelev A.S., Chudinov A.V. // Biophysics. 2018. V. 63. P. 512–518. https://doi.org/10.1134/S0006350918040127
Jena R.K., Yue C.Y. // Biomicrofluidics. 2012. V. 6. P. 012 822. https://doi.org/10.1063/1.3682098
Barsky V., Perov A., Tokalov S., Chudinov A., Kreindlin E., Sharonov A., Kotova E., Mirzabekov A. // J. Biomol. Screen. 2002. V. 7. P. 247–257. https://doi.org/10.1177/108705710200700308
Lysov Y., Barsky V., Urasov D., Urasov R., Cherepanov A., Mamaev D., Yegorov Y., Chudinov A., Surzhikov S., Rubina A., Smoldovskaya O., Zasedatelev A. // Biomed. Optics Express. 2017. V. 8. P. 4798–4810. https://doi.org/10.1364/BOE.8.004798