Isoforms of the cytoskeletal lim-domain protein zyxin in the early embryogenesis of Xenopus laevis
- Авторлар: Ivanova E.1, Parshina E.2, Zaraisky A.2, Martynova N.2
-
Мекемелер:
- Pirogov Russian National Research Medical University
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
- Шығарылым: Том 50, № 3 (2024)
- Беттер: 287-294
- Бөлім: Articles
- URL: https://journals.rcsi.science/0132-3423/article/view/261477
- DOI: https://doi.org/10.31857/S0132342324030077
- EDN: https://elibrary.ru/NZUWKW
- ID: 261477
Дәйексөз келтіру
Аннотация
Zyxin is a conserved mechanosensitive LIM-domain protein that regulates the assembly of F-actin filaments at cell junctions. At the same time, under mechanical stress, it can move from focal adhesions to stress fibrils and into the nucleus and affect gene expression. In Xenopus laevis embryonic cells, western blots with antibodies against Zyxin’s N-terminal and C-terminal LIM-domain regions revealed two full-length and two short isoforms. The intracellular localization of these isoforms and the number depending on the stage of embryo development were determined. According to our study, full forms with different electrophoretic mobility are localized differently in the cell, and the shortest isoform containing LIM-domains is stable during development, mainly located in the nucleus, and participates in gene expression regulation. This study may be of great value to understanding how the LIM-domain mechanotransducer proteins jointly influence morphogenesis and differentiation in vertebrates at early stages of development.
Негізгі сөздер
Толық мәтін
Авторлар туралы
E. Ivanova
Pirogov Russian National Research Medical University
Email: martnat61@gmail.com
Ресей, ul. Ostrovitianova 1, Moscow, 117997
E. Parshina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: martnat61@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 117997
A. Zaraisky
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: martnat61@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 117997
N. Martynova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Хат алмасуға жауапты Автор.
Email: martnat61@gmail.com
Ресей, ul. Miklukho-Maklaya 16/10, Moscow, 117997
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