Synthesis and Transfection Efficiency of Disulfide Policationic Amphiphiles
- Authors: Petukhov I.А.1, Puchkov P.А.1, Morozova N.G.1, Zenkova М.А.2, Maslov М.А.1
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Affiliations:
- Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
- Institute of Chemical Biology and Fundamental Medicine SB RAS
- Issue: Vol 49, No 2 (2023)
- Pages: 165-177
- Section: Articles
- URL: https://journals.rcsi.science/0132-3423/article/view/145876
- DOI: https://doi.org/10.31857/S0132342323010232
- EDN: https://elibrary.ru/GIHSNO
- ID: 145876
Cite item
Abstract
The synthesis of new polycationic amphiphiles containing a disulfide group in their structure has been carried out. Cationic liposomes were formed on the basis of the obtained compounds and helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine, which demonstrated the absence of toxicity to HEK293 and HeLa cells and high delivery efficiency of fluorescently labeled oligodeoxyribonucleotide. The efficiency of plasmid DNA delivery depended on the cell line and structure of the amphiphile, with liposomes based on tetracationic amphiphiles being the most effective transfectants. These liposomes may be used for in vitro transfection of eukaryotic cells as well as for further in vivo biological tests.
About the authors
I. А. Petukhov
Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
Email: puchkov_pa@mail.ru
Russia, 119571, Moscow, prosp. Vernadskogo 86
P. А. Puchkov
Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
Author for correspondence.
Email: puchkov_pa@mail.ru
Russia, 119571, Moscow, prosp. Vernadskogo 86
N. G. Morozova
Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
Email: puchkov_pa@mail.ru
Russia, 119571, Moscow, prosp. Vernadskogo 86
М. А. Zenkova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Email: puchkov_pa@mail.ru
Russia, 630090, Novosibirsk, prosp. Akad. Lavrentyeva 8
М. А. Maslov
Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
Author for correspondence.
Email: puchkov_pa@mail.ru
Russia, 119571, Moscow, prosp. Vernadskogo 86
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