Intrinsically Disordered Regions Affect the Expression of Toll-Like Receptor TIR Domains in Soluble Form

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Abstract

Here we investigate the effect of the C-terminal region on the solubility of TLR4 and TLR10 TIR domains upon the heterologous expression in Escherichia coli. We demonstrated that the absence of this region significantly enhances the yields of globular domains in a soluble form. These findings complement the protocols, previously established to synthesize the TLR TIR domains for the structural studies by various techniques, including by NMR spectroscopy.

About the authors

V. A. Lushpa

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Moscow, 142290 Russia

M. V. Goncharuk

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Moscow, 142290 Russia

A. S Arseniev

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Moscow, 142290 Russia

K. S. Mineev

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt

Moscow, 142290 Russia; Frankfurt am Main, 60438 Germany

S. A. Goncharuk

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: ms.goncharuk@gmail.com
Moscow, 142290 Russia

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