Combined Effects of Senexin B and Antitumour Agents on Neuroblastoma and Glioblastoma Cell Lines

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Abstract

Neurogenic tumors such as neuroblastoma and glioblastoma are highly heterogeneous and particularly aggressive: they are characterized by rapid growth, metastasis and resistance to treatment. Both tumors exhibit MYCN oncogene copy-number disruption, impaired gene transcription, and overall high transcriptional deregulation. In this study, we evaluated the survival of glioblastoma and neuroblastoma cells and performed real-time PCR analysis to assess the change in expression of MYCN, HAND2, PHOX2A, and PHOX2B oncogenes after exposure to senecin B in combination with temozolomide and the potential therapeutic agent 10058-F4. As a result, a trend of increased PHOX2B gene expression and decreased PHOX2A gene expression after drug exposure was observed in one of the neuroblastoma lines and both glioblastoma lines, an increase in MYCN and HAND2 gene expression was also observed. Viability tests showed that substance 10058-F4 was effective against neuroblastoma line but not glioblastoma lines. However, senexin B enhanced the inhibitory effect of 10058-F4 on glioblastoma cell lines and also enhanced the effect of temozolomide on the T98G cell line.

About the authors

D. V. Mazur

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, 117997 Russia

S. S. Pogodaeva

ITMO University

St. Petersburg, 197101 Russia

O. A. Kuchur

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS; ITMO University

Moscow, 117997 Russia; St. Petersburg, 197101 Russia

O. O. Miletina

ITMO University

St. Petersburg, 197101 Russia

A. I. Rezekina

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, 117997 Russia

E. G. Petrosyan

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, 117997 Russia

D. A. Rudik

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, 117997 Russia

E. I. Ivanova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, 117997 Russia

A. A. Shtil

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS; N.N. Blokhin National Medical Research Centre for Oncology

Moscow, 117997 Russia; Moscow, 115522 Russia

N. V. Antipova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS; National Research University “Higher School of Economics”

Email: nadine.antipova@gmail.com
Moscow, 117997 Russia; Moscow, 101000 Russia

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