Nano-frfast: design of a new genetically-encoded far-red fluorescent label
- 作者: Sokolinskaya E.L.1,2, Bogdanova Y.A.1,2, Myasnyanko I.N.2, Sokolov A.I.1,2, Krasnova S.A.1, Baranov M.S.1,2
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隶属关系:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
- Pirogov Russian National Research Medical University
- 期: 卷 51, 编号 1 (2025)
- 页面: 144-152
- 栏目: ПИСЬМА РЕДАКТОРУ
- URL: https://journals.rcsi.science/0132-3423/article/view/285933
- DOI: https://doi.org/10.31857/S0132342325010131
- EDN: https://elibrary.ru/LYMUUX
- ID: 285933
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详细
We propose a new fluorogen-activating protein nano-frFAST with only 98 amino acids based on a combination of the previously created fluorogen-activating proteins nanoFAST and frFAST. A series of fluorogens with an increased system of conjugated bonds, which are potentially capable of binding to this protein, were synthesized. Based on the results of the study, a promising fluorogen which is (Z)-5-((E)-3-(4-hydroxy-2,5-dimethoxyphenyl)allylidene)-2-thioxothiazolidin-4-one (HPAR-DOM) was identified. We demonstrate that the nano-frFAST–HPAR-DOM complex can be used as a genetically encoded far-red fluorescent label for staining individual structures of living cells.
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作者简介
E. Sokolinskaya
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University
编辑信件的主要联系方式.
Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow
Yu. Bogdanova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University
Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow
I. Myasnyanko
Pirogov Russian National Research Medical University
Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow
A. Sokolov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University
Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow
S. Krasnova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow
M. Baranov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University
Email: svetlanakr2002@mail.ru
俄罗斯联邦, Moscow; Moscow
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