Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products
- Авторлар: Shershov V.1, Kuznetsova V.1, Zasedatelev A.1, Vasiliskov V.1, Shekalova E.1, Shtylev G.1, Shishkin I.1, Levashova A.1, Lapa S.1, Chudinov A.1
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Мекемелер:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Шығарылым: Том 49, № 6 (2023)
- Беттер: 649-656
- Бөлім: ПИСЬМА РЕДАКТОРУ
- URL: https://journals.rcsi.science/0132-3423/article/view/148164
- DOI: https://doi.org/10.31857/S0132342323050056
- EDN: https://elibrary.ru/BAKVTC
- ID: 148164
Дәйексөз келтіру
Аннотация
The synthesis of fluorescently labeled nucleotides containing a zwitterionic indodicarbocyanine dye attached via a trans-alkene spacer at the C5 position of the pyrimidine base was carried out, and their substrate efficiency was tested under conditions of recombinase polymerase amplification (RPA). As a result of RPA, the formation of full-sized target products of the ebpS gene fragment of the causative agent of bacterial pneumonia (Staphylococcus aureus) and a high density of fluorescent label embedding occurred.
Авторлар туралы
V. Shershov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
V. Kuznetsova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
A. Zasedatelev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
V. Vasiliskov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
E. Shekalova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
G. Shtylev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
I. Shishkin
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
A. Levashova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
S. Lapa
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
A. Chudinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: shershov@list.ru
Russia, 119991, Moscow, ul. Vavilova 32
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