Synthesis of Liposomes Conjugated with CpG Oligonucleotide and Loaded with a Set of T-Cell Epitopes of the SARS-CoV-2 Virus

D. S. Tretiakova; Третьякова Д. С.; T. L. Azhikina; Ажикина Т. Л.; I. A. Boldyrev; Болдырев И. А.; E. V. Svirshchevskaya; Свирщевская Е. В.; E. L. Vodovozova; Водовозова Е. Л.

doi:10.31857/S0132342323040437

Synthesis of Liposomes Conjugated with CpG Oligonucleotide and Loaded with a Set of T-Cell Epitopes of the SARS-CoV-2 Virus

Authors: Tretiakova D.S.¹, Azhikina T.L.¹, Boldyrev I.A.¹, Svirshchevskaya E.V.¹, Vodovozova E.L.¹
Affiliations:
1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Issue: Vol 49, No 4 (2023)
Pages: 434-440
Section: ПИСЬМА РЕДАКТОРУ
URL: https://journals.rcsi.science/0132-3423/article/view/139182
DOI: https://doi.org/10.31857/S0132342323040437
EDN: https://elibrary.ru/OEDGQI
ID: 139182

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Abstract
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Abstract

The synthesis of lipid conjugate of immunostimulatory oligodeoxyribonucleotide CpG-ODN (PD-CpG-DOPE) is described. Liposomes loaded with a composition of T-cell epitopes of the SARS-CoV-2 virus (7 peptides) and carrying PD-CpG-DOPE conjugate in the membrane, including lyophilized liposomes suitable for long-term storage, were prepared. In vitro experiments on mouse peritoneal exudate cells showed a tendency to increase the immunogenicity of liposomes with peptides when PD-CpG-DOPE conjugate was introduced into the lipid bilayer, compared with the addition of the (commercial) phosphorothioate derivative of CpG-ODN in solution.

Keywords

Т-cells, epitopes, SARS-CoV-2, vaccines, peptides, liposomes, CpG-ODN

References

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