Impairment of the Viability of Transformed Chinese Hamster Cells in a Nonsubcultured Culture under the Influence of Exogenous Oxidized Guanoside is Manifested Only in the Stationary Phase of Growth
- Authors: Morgunova G.V.1, Klebanov A.A.1
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Affiliations:
- Evolutionary Cytogerontology Sector, School of Biology
- Issue: Vol 73, No 3 (2018)
- Pages: 124-129
- Section: Gerontology
- URL: https://journals.rcsi.science/0096-3925/article/view/173729
- DOI: https://doi.org/10.3103/S0096392518030136
- ID: 173729
Cite item
Abstract
Despite the fact that oxidation products of nucleotides and nucleosides are markers of oxidative stress, reports of the paradoxical ability of these compounds to protect cells from the harmful effects of reactive oxygen species began to appear more often. Among all nitrogenous bases, guanine is most susceptible to the influence of oxidative stress; therefore, guanosine is oxidized more often than other bases. In the present work, the effect of exogenous 8-oxo-2′-deoxyguanosine on the growth and “stationary phase aging” (accumulation of “age-related” changes in cultured cells during cell proliferation slowing down within a single passage and subsequent “aging” in the stationary growth phase) of nonsubcultured transformed Chinese hamster cells was studied. We showed that the nucleoside is rapidly absorbed by the cells from the medium, but it does not affect the growth of the culture, and impairs the viability of the cells in the late stationary growth phase. Thus, no mitogenic or geroprotective effect of 8-oxo-2′-deoxyguanosine was found.
About the authors
G. V. Morgunova
Evolutionary Cytogerontology Sector, School of Biology
Author for correspondence.
Email: morgunova@mail.bio.msu.ru
Russian Federation, Moscow, 119234
A. A. Klebanov
Evolutionary Cytogerontology Sector, School of Biology
Email: morgunova@mail.bio.msu.ru
Russian Federation, Moscow, 119234